DOI: 10.1101/493098Dec 11, 2018Paper

Viperin binds STING and enhances the type-I interferon response following dsDNA detection

BioRxiv : the Preprint Server for Biology
K M CrosseKarla J. Helbig

Abstract

Viperin is an interferon-inducible protein that is pivotal for eliciting an effective immune response against an array of diverse viral pathogens. Here we describe a mechanism of viperin's broad antiviral activity by demonstrating the protein's ability to synergistically enhance the innate immune dsDNA signalling pathway to limit viral infection. Viperin co-localised with the key signalling molecules of the innate immune dsDNA sensing pathway, STING and TBK1; binding directly to STING and inducing enhanced K63-linked polyubiquitination of TBK1. Subsequent analysis identified viperin's necessity to bind the cytosolic iron-sulphur assembly component 2A, to prolong its enhancement of the type-I interferon response to aberrant dsDNA. Here we show that viperin facilitates the formation of a signalling enhanceosome, to coordinate efficient signal transduction following activation of the dsDNA signalling pathway; which results in an enhanced antiviral state. We also provide evidence for viperin's radical SAM enzymatic activity as an intrinsic self-limiting function of its immunomodulatory functions. This data further defines viperin's role as a positive regulator of innate immune signalling, offering a mechanism of viperin's broad ant...Continue Reading

Related Concepts

Antiviral Agents
DNA
Hepatitis B
Hepatitis B Virus
Interferons
Esthesia
Signal Pathways
Signal Transduction
Viral Proteins
Virus Diseases

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

© 2021 Meta ULC. All rights reserved