PMID: 7580916Apr 1, 1995Paper

Vir typing: a long-PCR typing method for group A streptococci

PCR Methods and Applications
D GardinerK S Sriprakash

Abstract

We have developed a new procedure (Vir typing) for typing Streptococcus pyogenes, by amplifying the entire 5- to 7-kb variable vir regulon by long PCR. The amplified DNA is then cleaved with HaeIII and visualized by ethidium bromide fluorescence after agarose gel electrophoresis. A simple procedure for preparing DNA of sufficiently high quality from 96 samples was employed simultaneously. This DNA was also used to develop a random amplified polymorphic DNA (RAPD) procedure. The discriminatory power of the two DNA-based procedures was compared with previous methods, M typing, and multilocus enzyme electrophoresis. Both procedures were highly discriminatory, but the stoichiometric yield of restriction fragments in Vir typing allows unambiguous interpretation of results.

Citations

Mar 12, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D J McMillanK S Sriprakash
Aug 1, 1997·Veterinary Parasitology·A E LewR E Bock
Feb 5, 2002·Journal of Clinical Microbiology·Luca A VitaliSandro Ripa
Dec 10, 2003·Journal of Clinical Microbiology·Rebecca J TowersGursharan S Chhatwal
Apr 12, 2003·Journal of Clinical Microbiology·Claudia M BrandtRudolf Lütticken
Mar 23, 1999·Archives of Disease in Childhood·J R Carapetis, B J Currie
Dec 12, 2001·American Journal of Respiratory and Critical Care Medicine·V BandiS B Greenberg
May 17, 2005·Microbes and Infection·Mark R DaviesKadaba S Sriprakash
Mar 25, 2004·FEMS Immunology and Medical Microbiology·David J McMillanKadaba S Sriprakash
Nov 26, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L J RichardsonP M Giffard
Feb 13, 2015·Critical Reviews in Food Science and Nutrition·M MoslemiAmir M Mortazavian
Mar 13, 2010·Microbiological Research·J Rajendhran, P Gunasekaran
Jun 11, 2004·Letters in Applied Microbiology·V SagarA Chakraborti
Feb 24, 2001·Cellular Microbiology·G MolinariG S Chhatwal
Feb 6, 2004·Expert Review of Vaccines·Colleen OliveIstvan Toth
Oct 18, 2008·Journal of Medical Microbiology·Bogdan Luca-HarariAftab Jasir
Nov 13, 2009·Expert Review of Vaccines·Pierre R SmeestersMelina M Georgousakis
Aug 23, 2008·Journal of Medical Microbiology·Charmaine LloydThangam Menon
Aug 2, 2002·The Pediatric Infectious Disease Journal·Tania M Shelby-JamesJohn D Mathews
Dec 23, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nilay DeyHarpreet Vohra
Mar 8, 2003·Journal of Clinical Microbiology·Claudia ZampaloniSandro Ripa
May 9, 1998·Journal of Clinical Microbiology·J HartasK S Sriprakash
May 23, 1998·Journal of Clinical Microbiology·D L GardinerK S Sriprakash

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.