PMID: 6397935Jan 1, 1984Paper

Viral antigen in endocrine cells of the pancreatic islets and adrenal cortex of Pekin ducks infected with duck hepatitis B virus

Virus Research
M S HalpernJ M England

Abstract

Endocrine cells in the pancreas and adrenal glands of duck hepatitis B virus-infected ducks were examined for the presence of viral antigen. Analysis of pancreas tissue was based on double immunofluorescence assays in which anti-duck hepatitis B virus serum was used to detect viral antigen, and anti-glucagon and anti-insulin serum were used, respectively, to identify endocrine alpha and beta cells. Assays of pancreas from infected ducks ranging in age from 3 to 20 weeks indicated that subpopulations of both alpha and beta cells expressed viral antigen. A higher percentage of viral antigen-positive cells was observed in alpha-islets than in beta-islets. As assayed by immunofluorescence and immunoperoxidase staining with anti-viral serum, small clusters of viral antigen-positive cells were detected in the adrenal glands of young infected ducks. These cells were identified as cortical cells on the basis of histologic criteria.

References

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Citations

Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·C Seeger, W S Mason
May 28, 2011·The Journal of Comparative Neurology·Laurent GautronJoel K Elmquist
Mar 12, 2015·Virology·Christoph Seeger, William S Mason
Apr 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W S Mason, J M Taylor
May 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J S FreimanY E Cossart
Feb 1, 2012·International Journal of Endocrinology·Louiza BelkacemiWilly J Malaisse
Mar 1, 1987·Journal of Virology·M S HalpernJ M England

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