Viral antigens detectable in CSF exosomes from patients with retrovirus associated neurologic disease: functional role of exosomes

Clinical and Translational Medicine
Monique R AndersonSteven Jacobson

Abstract

HTLV-1 infects over 20 million people worldwide and causes a progressive neuroinflammatory disorder in a subset of infected individuals called HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP). The detection of HTLV-1 specific T cells in the cerebrospinal fluid (CSF) suggests this disease is immunopathologically mediated and that it may be driven by viral antigens. Exosomes are microvesicles originating from the endosomal compartment that are shed into the extracellular space by various cell types. It is now understood that several viruses take advantage of this mode of intercellular communication for packaging of viral components as well. We sought to understand if this is the case in HTLV-1 infection, and specifically if HTLV-1 proteins can be found in the CSF of HAM/TSP patients where we know free virus is absent, and furthermore, if exosomes containing HTLV-1 Tax have functional consequences. Exosomes that were positive for HTLV-1 Tax by Western blot were isolated from HAM/TSP patient PBMCs (25/36) in ex vivo cultures by trapping exosomes from culture supernatants. HTLV-1 seronegative PBMCs did not have exosomes with Tax (0/12), (Fisher exact test, p = 0.0001). We were able to observe HAM/TSP patient CSF (...Continue Reading

References

Nov 1, 1990·Journal of Virology·J H RichardsonA G Dalgleish
Jan 1, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·S Jacobson
May 14, 1999·Immunology Today·D WodarzC R Bangham
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D F DukersJ M Middeldorp
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nicolas BlanchardClaire Hivroz
Nov 12, 2002·Nature Immunology·Clotilde ThérySebastian Amigorena
Jun 8, 2004·The Journal of Infectious Diseases·Peter K C GoonCharles R M Bangham
Jan 22, 2005·The Journal of Biological Chemistry·Timothy AlefantisBrian Wigdahl
May 3, 2005·The Journal of Clinical Investigation·Yoshihisa YamanoSteven Jacobson
Sep 13, 2005·Oncogene·Fernando A ProiettiEdward L Murphy
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rebecca D Wiley, Suryaram Gummuluru
Apr 6, 2006·The Journal of Clinical Investigation·David SibonEric Wattel
Jun 9, 2006·European Journal of Immunology·Charlotte AdmyreSusanne Gabrielsson
Jun 27, 2006·Journal of Cellular and Molecular Medicine·Xiao-Bo LiShun-Qing Xu
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elodie SeguraClotilde Théry
Sep 15, 2007·Journal of Virology·Shuo LiUlf Dittmer
Jun 28, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Allan R TunkelUNKNOWN Infectious Diseases Society of America
Aug 5, 2008·Journal of Immunological Methods·Réjean CantinCaroline Gilbert
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Veronique LepoutreZafar K Khan
May 28, 2010·Journal of Clinical and Experimental Hematopathology : JCEH·Yorifumi Satou, Masao Matsuoka
Jun 25, 2010·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Shuji Izumo
Oct 26, 2011·The International Journal of Biochemistry & Cell Biology·Anna-Kristin Ludwig, Bernd Giebel
Feb 7, 2012·Journal of Neuroimaging : Official Journal of the American Society of Neuroimaging·Iordanis E EvangelouSteven Jacobson
Jun 23, 2012·Pharmacological Reviews·Edwin van der PolRienk Nieuwland
Aug 28, 2012·Advanced Drug Delivery Reviews·Samir El AndaloussiMatthew J A Wood
Oct 31, 2012·Frontiers in Microbiology·Claudine Pique, Kathryn S Jones
Dec 12, 2012·Frontiers in Microbiology·Robert CurrerFatah Kashanchi
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Apr 23, 2013·Reviews in Medical Virology·Yorifumi Satou, Masao Matsuoka
May 11, 2013·The Journal of Biological Chemistry·Aarthi NarayananFatah Kashanchi

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Citations

Jul 2, 2020·Viruses·Naseer A KutchyShilpa Buch
May 21, 2019·Frontiers in Microbiology·Satoshi Nozuma, Steven Jacobson
Sep 24, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kyriaki BakirtziCharalabos Pothoulakis
Dec 1, 2019·Retrovirology·Yoshimi Enose-Akahata, Steven Jacobson
Jul 18, 2020·Veterinary Research·Guowei XuXiangtao Liu
Dec 3, 2020·Journal of Experimental Pharmacology·Robert A NewmanRobert Harrod
Feb 12, 2021·Viruses·Liying ZhangLinzhu Ren
Jun 3, 2021·Pharmaceutics·Dmitry PolyakovTatiana Tennikova
Aug 8, 2021·International Journal of Molecular Sciences·Greta ForlaniMaria Grazia Romanelli

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Methods Mentioned

BETA
ELISA
flow cytometry
pulldown
pulldowns
PCR
biopsy
Assay
Enzyme Linked Immunosorbent Assay
FACS
light scattering

Software Mentioned

GraphPad Prism
Nanosight
GraphPad QuickCalcs

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