Viral contamination of embryos cryopreserved in liquid nitrogen

Cryobiology
A BielanskiC Lutze-Wallace

Abstract

Despite the worldwide application of embryo-freezing technology as the means of preserving germplasm of mammalian species, there is no information available on the possible transmission of infectious agents to cryopreserved embryos via contaminated liquid nitrogen (LN). Recently, it has been reported that new methods of cryopreservation which employ ultrarapid freezing or vitrification require direct contact between the freezing medium containing oocytes or embryos and liquid phase nitrogen (LPN). As models for human and animal viral pathogens three bovine viruses, bovine viral diarrhea virus (BVDV), bovine herpesvirus-1 (BHV), and bovine immunodeficiency virus (BIV), were employed to study the potential for their transmission by experimentally contaminated LN to embryos frozen and stored in open freezing containers. Bovine embryos in a mixture of 20% ethylene glycol, 20% ME(2)SO, and 0.6% sucrose were vitrified in either unsealed standard 0.25 ml or modified open pulled straws or in plastic cryovials and then plunged into contaminated LPN. After 3-5 weeks of storage in LN, embryos were thawed and sequentially washed and only those with intact ZP were pooled together and tested in batches of three for viral contamination. From ...Continue Reading

References

Oct 1, 1989·The British Journal of Dermatology·S K Jones, J M Darville
Sep 1, 1968·Journal of Virology·C Wallis, J L Melnick
Nov 1, 1971·JAMA : the Journal of the American Medical Association·C R Charles, D J Sire
Jul 15, 1995·Lancet·R S TedderK G Patterson
Apr 8, 1995·Lancet·W E CarrollM L Ellis
May 27, 1995·Lancet·S K JonesJ L Burton
May 1, 1996·Biology of Reproduction·A MartinoS P Leibo

❮ Previous
Next ❯

Citations

Feb 4, 2010·Journal of Assisted Reproduction and Genetics·Rikikazu SugiyamaMasato Inoue
Nov 9, 2010·Journal of Assisted Reproduction and Genetics·Nina DesaiTommaso Falcone
Jun 12, 2012·Journal of Assisted Reproduction and Genetics·Xing-ling WangHui-rong Shi
Jan 29, 2013·Journal of Assisted Reproduction and Genetics·N De MunckH Van de Velde
Jun 11, 2008·Human Reproduction·Jeong Hee MoonHae Kwon Kim
Nov 22, 2013·Reproductive Biology and Endocrinology : RB&E·Yuan ChenPing Liu
May 16, 2013·Reproductive Biology and Endocrinology : RB&E·Nina N DesaiTommaso Falcone
Jul 9, 2011·PloS One·L Gabriel Sanchez-PartidaJustin C St John
Feb 27, 2007·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·William F SwansonSuzanne Kennedy-Stoskopf
May 15, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·V IsachenkoJ M Weiss
Aug 19, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Y H ChoiK Hinrichs
Apr 30, 2014·BioMed Research International·János KoncSándor Cseh
Oct 24, 2014·Biopreservation and Biobanking·Michael RaminJohannes Schenkel
Feb 18, 2016·Biopreservation and Biobanking·Phillip M Baird, Elaine W Gunter
Aug 7, 2009·Human Fertility : Journal of the British Fertility Society·UNKNOWN Association of Clinical Embryologists and British Fertility SocietyRichard Anderson
Sep 10, 2014·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Zaynab GamzatovaEvgenia Kalinina
Dec 25, 2008·Expert Review of Medical Devices·Weon-Young Son, Seang Lin Tan
Jan 10, 2012·Reproductive Biomedicine Online·Dominic StoopPaul Devroey
Jan 31, 2012·Reproductive Biomedicine Online·Yuji EndoHiroaki Funahashi
Aug 17, 2011·Reproductive Biomedicine Online·L ParmegianiM Filicori
Nov 9, 2010·Taiwanese Journal of Obstetrics & Gynecology·Tseng-Kai LinHsiao-Ching Lo
Mar 17, 2011·Fertility and Sterility·Krinos M TrokoudesXiao Zhang
Jan 29, 2011·Fertility and Sterility·Enrique CriadoPaolo Emanuele Levi-Setti
May 20, 2011·Reproductive Biomedicine Online·Jeffrey Boldt
Jan 29, 2011·Reproductive Biomedicine Online·Alessio PaffoniGuido Ragni
Sep 4, 2008·Reproductive Biomedicine Online·James J StacheckiJacques Cohen
Aug 29, 2009·Reproductive Biomedicine Online·R FadiniM B Dal Canto
Mar 16, 2007·Reproductive Biomedicine Online·Safaa Al-HasaniAskan Schultze-Mosgau
Jan 30, 2004·Reproductive Biomedicine Online·Juergen LiebermannMichael J Tucker
Aug 31, 2004·Reproductive Biomedicine Online·D Mortimer
Feb 9, 2010·Fertility and Sterility·Ana CoboJosé Remohí

❮ Previous
Next ❯