Viral contribution to dissolved DNA in the marine environment as determined by differential centrifugation and kingdom probing.

Applied and Environmental Microbiology
S C Jiang, John H Paul

Abstract

Dissolved or filterable (<0.2-(mu)m-pore-size filter) DNA is a ubiquitous component of the dissolved organic matter in the surface waters of this planet. In an effort to understand the composition and possible sources, we subjected dissolved DNA concentrated by vortex flow filtration from offshore and coastal environments to differential centrifugation and probing with 16S rRNA-targeted kingdom oligonucleotide probes. Initial studies with calf thymus soluble DNA and T2 phage particles indicated that high-speed ultracentrifugation (201,000 x g for 90 min), a method to separate viral particles from soluble DNA used by other investigators, resulted in pelleting of nearly all the DNA and virus particles. Lower-speed centrifugation (11,200 to 25,800 x g for 90 min) resulted in >99% of the virus particles being collected in the pellet and (equiv)65% of the calf thymus DNA remaining in the supernatant. Employing this approach, we estimate that approximately 50% of the filterable DNA from marine environments is truly soluble or free DNA and that the other half is composed of bound forms (viral particles and, potentially, colloids). Of the bound form, 17 to 30% could be accounted for by viral particles, by calculating the amount of vira...Continue Reading

References

Jan 1, 1975·Methods in Cell Biology·D R CryerJ Marmur
Oct 1, 1990·Applied and Environmental Microbiology·J H PaulJ P Cannon
Aug 1, 1991·Applied and Environmental Microbiology·J H PaulJ B Rose
Jun 1, 1990·Applied and Environmental Microbiology·J H PaulJ Thurmond
May 3, 1990·Nature·S J GiovannoniK G Field
May 1, 1989·Journal of Bacteriology·D W DorwardR C Judd
Aug 1, 1989·Journal of Bacteriology·D W Dorward, C F Garon
Aug 1, 1989·Applied and Environmental Microbiology·J H Paul, A W David
Aug 10, 1989·Nature·O BerghM Heldal
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M L Pifer, H O Smith
May 1, 1988·Applied and Environmental Microbiology·D A StahlL Montgomery
Jan 1, 1987·Applied and Environmental Microbiology·J H PaulM F DeFlaun
Apr 1, 1982·Applied and Environmental Microbiology·J H Paul
Jun 1, 1982·Applied and Environmental Microbiology·J H Paul, B Myers
Oct 1, 1986·Applied and Environmental Microbiology·M F DeflaunD Davis
Jun 1, 1990·Applied and Environmental Microbiology·D W Dorward, C F Garon
Mar 1, 1993·Applied and Environmental Microbiology·A MaruyamaT Higashihara
Dec 1, 1993·Applied and Environmental Microbiology·M G WeinbauerP Peduzzi

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Citations

Sep 30, 2009·PloS One·Fabrice NotRamon Massana
Jan 18, 2015·Applied and Environmental Microbiology·Cátia CarreiraCorina P D Brussaard
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·K E Wommack, R R Colwell
Apr 9, 2005·The Journal of Eukaryotic Microbiology·Kathryn J Coyne, S Craig Cary
Oct 27, 2007·Environmental Biosafety Research·Kaare M NielsenDaniele Daffonchio
Mar 31, 2012·Annual Review of Marine Science·Mya Breitbart
Jun 1, 2000·Applied and Environmental Microbiology·Y BettarelH Laveran
Mar 18, 2008·Applied and Environmental Microbiology·Danielle M Winget, K Eric Wommack
Jun 3, 2005·Nature Reviews. Microbiology·Eric E Allen, Jillian F Banfield
Sep 9, 2006·Environmental Microbiology·Linda WegleyForest Rohwer
Aug 12, 2017·Frontiers in Microbiology·Alejandra L Ibáñez de AldecoaJosé E González-Pastor
Mar 18, 2017·Viruses·Flavia FlavianiEdward P Rybicki
Feb 9, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Thanh H Nguyen, Menachem Elimelech
Nov 10, 2009·Trends in Microbiology·Thomas SchoenfeldDavid Mead
Dec 3, 2014·Water Research·M R BrownT P Curtis

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