Viral delivery of heme oxygenase-1 attenuates photoreceptor apoptosis in an experimental model of retinal detachment

Vision Research
Mong-Ping ShyongYeou-Ping Tsao

Abstract

This study was designed to evaluate the efficacy of subretinal injection of recombinant adeno-associated virus vector expressing heme oxygenase-1 (rAAV-HO-1) in attenuating photoreceptor apoptosis induced by experimental retinal detachment (RD) in Sprague-Dawley rats. Our results disclosed that subretinal rAAV-HO-1 delivery achieved localized high HO-1 gene expression in retinal outer nuclear layer (ONL) compared with rAAV-lacZ-injected eyes and eyes with RD left untreated both at 2 (p=0.003) and 28 (p=0.007) days of RD. The ONL thickness (p=0.018) and mean photoreceptor nuclei count (p=0.009) in eyes receiving rAAV-HO-1 injection was significantly higher than in rAAV-lacZ-injected or eyes with RD left untreated at 28 days of RD. There were fewer apoptotic photoreceptor nuclei at 2 (p=0.008) and 5 (p=0.018) days of RD and less activated caspase-3 expression (p=0.008) at 2 days of RD in rAAV-HO-1 treated eyes than in control eyes. These data supported that gene transfer approach might attenuate photoreceptor apoptosis caused by RD with a resultant better ONL preservation.

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Citations

Jun 23, 2012·Current Eye Research·Jun ZhaoDing Sun
Apr 21, 2011·Journal of Cellular Physiology·Adna HalilovicMichal Laniado Schwartzman
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Mar 16, 2017·Frontiers in Cellular Neuroscience·Lin ChengMengqing Xiang
Jul 22, 2018·Current Eye Research·Basma BaccoucheRafika Ben Chaouacha-Chekir
Feb 5, 2021·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Alejandra DaruichFrancine Behar-Cohen

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