PMID: 9420282Jan 7, 1998Paper

Viral E6-E7 transcription in the basal layer of organotypic cultures without apparent p21cip1 protein precedes immortalization of human papillomavirus type 16- and 18-transfected human keratinocytes

Journal of Virology
Renske D M SteenbergenL T Chow

Abstract

Organotypic cultures of human keratinocytes provide a useful model system to study human papillomavirus (HPV)-host cell interactions. In this study, we analyzed organotypic cultures of two HPV type 16 (HPV16) (FK16A and FK16B)- and two HPV18 (FK18A and FK18B)-transfected keratinocyte cell lines through the process of immortalization in vitro. For FK16A and FK18B cells, passages of both mortal cells in their extended life span and subsequent immortal stages were studied. Mortal cells of FK16A and FK18B showed a morphology reminiscent of mild to moderate dysplasia, whereas in their immortal descendants, severely dysplastic features were observed. Immortal FK18A cells were mildly to moderately dysplastic, while FK16B cells were severely dysplastic. The increasing degrees of dysplasia were associated with a decreasing expression of differentiation markers cytokeratin 10 and profilaggrin. All raft cultures expressed E6-E7 mRNAs in the basal layer, while the amount of viral transcripts in the suprabasal cells was in general proportional to the degree of dysplasia. In all cases, E6-E7 transcription and dysplastic features were highly correlated with cellular proliferation, as assessed by Ki-67 (MIB-1) antigen expression. Moreover, hig...Continue Reading

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Citations

May 14, 1998·International Journal of Cancer. Journal International Du Cancer·R D SteenbergenP J Snijders
May 19, 2005·International Journal of Cancer. Journal International Du Cancer·Nicole W J BulkmansChris J L M Meijer
Oct 16, 2003·The Nurse Practitioner·Alana Heise
Feb 3, 2007·International Journal of Cancer. Journal International Du Cancer·Sarah F MartinC Simon Herrington
Nov 1, 2007·British Journal of Cancer·F E HenkenR D M Steenbergen
Aug 14, 2010·Transplantation·Rachael TurnerLola Reid
Jan 18, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Saskia M WiltingRenske D M Steenbergen
Feb 19, 2004·Journal of the National Cancer Institute·Renske D M SteenbergenPeter J F Snijders
Sep 29, 2004·Journal of Virology·Brian A Van TineLouise T Chow
Dec 20, 2005·The Journal of Pathology·Peter J F SnijdersChris J L M Meijer
Jun 18, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Louise T ChowBettie M Steinberg
Aug 21, 2015·Epigenetics : Official Journal of the DNA Methylation Society·Malin LandoHeidi Lyng
Oct 26, 2007·International Journal of Cancer. Journal International Du Cancer·Jillian de WildeRenske D M Steenbergen
Aug 8, 2009·The Journal of Pathology·Renée M OvermeerRenske D M Steenbergen
Jun 15, 2011·Molecular Cancer·Florianne E HenkenRenske D M Steenbergen

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