Viral inactivation of human platelet lysate by gamma irradiation preserves its optimal efficiency in the expansion of human bone marrow mesenchymal stromal cells

Transfusion
Sabrina ViauBruno Delorme

Abstract

Human platelet lysate (hPL) represents a powerful medium supplement for human mesenchymal stromal cell (hMSC) expansion. The recently published general chapters of the Pharmacopeia require the addition of a step of viral inactivation during the production process of such raw biological material used for cell-based medicinal products. The ability of gamma irradiation to inactivate viruses from a panel representative of the virus diversity was evaluated. The impact of gamma irradiation on hPL composition and efficiency as a supplement for hMSC culture was evaluated. An efficient inactivation of all the viruses tested was demonstrated, with the minimum reduction factors obtained being superior to 4.5 log10 for human immunodeficiency virus (HIV) and hepatitis A virus (HAV) and superior to 5 log10 for bovine viral diarrhea virus (BVDV), pseudorabies virus (PRV) and porcine parvovirus (PPV). The gamma irradiation did not affect the content in interesting biochemical factors for cell culture or in growth factors (GF), except to basic fibroblast GF (bFGF) whereas it highly impacted the contents in the factors involved in the coagulation cascade. Finally, gamma irradiated hPL remained as efficient as non-irradiated hPL for the prolifera...Continue Reading

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Citations

Jan 4, 2020·Transfusion·Johannes BlümelAlbert Stühler
Dec 1, 2020·Platelets·Lassina BarroThierry Burnouf
Nov 19, 2019·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Thierry BurnoufJerard Seghatchian
Feb 6, 2021·Regenerative Medicine·Vafa MeftahpourLeili Aghebati-Maleki
Jun 3, 2021·International Journal of Molecular Sciences·Michaela OellerKatharina Schallmoser
Aug 23, 2021·Advanced Drug Delivery Reviews·Simon StaubachBernd Giebel

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