Viral infection detection using metagenomics technology in six poultry farms of eastern China

PloS One
Yuan QiuKaicheng Wang

Abstract

With rapidly increasing animal pathogen surveillance requirements, new technologies are needed for a comprehensive understanding of the roles of pathogens in the occurrence and development of animal diseases. We applied metagenomic technology to avian virus surveillance to study the main viruses infecting six poultry farms in two provinces in eastern China. Cloacal/throat double swabs were collected from 60 birds at each farm according to a random sampling method. The results showed that the method could simultaneously detect major viruses infecting farms, including avian influenza virus, infectious bronchitis virus, Newcastle disease virus, rotavirus G, duck hepatitis B virus, and avian leukemia virus subgroup J in several farms. The test results were consistent with the results from traditional polymerase chain reaction (PCR) or reverse transcription-PCR analyses. Five H9N2 and one H3N8 avian influenza viruses were detected at the farms and were identified as low pathogenic avian influenza viruses according to HA cleavage sites analysis. One detected Newcastle disease virus was classified as Class II genotype I and avirulent type according to F0 cleavage sites analysis. Three avian infectious bronchitis viruses were identifie...Continue Reading

References

Jul 1, 1997·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·L Haas
Jan 29, 2002·Archives of Virology·E HoffmannD R Perez
Jun 29, 2002·Journal of Clinical Microbiology·Chi-Young J WangBruce F Smith
Dec 4, 2003·Microbial Pathogenesis·Aruna PandaSiba K Samal
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jun 25, 2004·Nucleic Acids Research·Scott McGinnis, Thomas L Madden
Jan 27, 2007·Genome Research·Daniel H HusonStephan C Schuster
Jun 20, 2008·Journal of Clinical Microbiology·Astrid GallMartin Beer
Aug 30, 2008·Sheng wu gong cheng xue bao = Chinese journal of biotechnology·Hosakatte Niranjana MurthyKee Yoeup Paek
Aug 7, 2009·Journal of Clinical Microbiology·Fei-Fei GePei-Hong Liu
Apr 5, 2011·Veterinary Microbiology·A O JenkinsJ Godfroid
Aug 1, 2012·Polish Journal of Veterinary Sciences·D A RoussanR H Al-Rifai
Dec 5, 2012·Electrophoresis·Barry MerrimanJonathan M Rothberg
Mar 15, 2013·Molecular Biology and Evolution·Barry G Hall
Aug 21, 2013·Forensic Science International. Genetics·Walther ParsonJodi Irwin
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Mar 1, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Wei WuShengwang Liu
Jun 10, 2015·Phytopathology·Marilyn J RoossinckPhilippe Roumagnac
Aug 8, 2015·Virology Journal·Jing-Yu WangHung-Jen Liu
Mar 8, 2017·Virus Research·Kai-Cheng WangJi-Ming Chen
Sep 25, 2017·International Journal of Molecular Sciences·Shu-Yi Lin, Hui-Wen Chen
Sep 24, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Liwen XuShengwang Liu

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Datasets Mentioned

BETA
SRR5367358
MH487502

Methods Mentioned

BETA
RNA-Seq
PCR
electrophoresis
chip

Software Mentioned

Rapid Typing and Analysis
BLASTx
Rapid Typing and Analysis Software of
CLC Genomics Workbench
BLAST
MEGAN
MetaGenome Analyzer MEGAN
Rapid Typing and Analysis Software
MUSCLE
Basic Local Alignment Search Tool ( BLASTx )

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