Viral infection. I. Regulation of protein synthesis during vaccinia viral infection of animal cells

Archives of Biochemistry and Biophysics
A BoseN K Gupta

Abstract

Regulation of vaccinia viral infection was studied using three animal cell lines: KRC-7 (rat hepatoma), L929 (mouse fibroblast), and CV-1 (African green monkey kidney). KRC-7 is highly enriched in p67, a glycoprotein which protects eIF-2 alpha-subunit from phosphorylation by eIF-2 kinases. We report: (i) At 5 pfu per cell of the virus, KRC-7 is resistant to the virus. Other cells are sensitive. At 25 pfu per cell of the virus, KRC-7 is also sensitive to the virus. After productive viral infection, the cell extracts showed strong p67-DG activity and actively deglycosylated exogenous p67. After p67-deglycosylation, the cell extracts also phosphorylated eIF-2. (ii) The rate of synthesis of a major host protein (approximately 45 kDa) in infected L929 cells measured after 2 h of viral infection declined more than 50%. The rate declined thereafter. The rate of synthesis of host proteins in viral-resistant KRC-7 cells (infected with 5 pfu per cell of the virus) remained unchanged. The mechanism of resistance of KRC7 cells to vacinia virus at 5 pfu per cell of the virus was investigated. The p67 level in these cells was varied by growing the cells under different physiological conditions such as serum starvation and expression of p67-s...Continue Reading

Citations

May 4, 2011·World Journal of Biological Chemistry·Shiyong Wu
Apr 17, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Bernhard Schaller, Rudolf Graf
Jan 26, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Collin R BrooksSalim I Khakoo
Aug 26, 1998·Biochemical and Biophysical Research Communications·M ChatterjeeN K Gupta
Oct 31, 2000·Journal of the Neurological Sciences·B C WhiteG S Krause
Sep 1, 2009·Biochimica Et Biophysica Acta·Bansidhar Datta

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