Viral infection. II. Hemin induces overexpression of p67 as it partially prevents appearance of an active p67-deglycosylase in baculovirus-infected insect cells

Archives of Biochemistry and Biophysics
D SahaN K Gupta

Abstract

The roles of p67-deglycosylase (p67-DG) in the regulation of protein synthesis in baculovirus-infected insect cells were studied. Like vaccinia viral infection, baculovirus infection of insect cells also induced the appearance of a p67-DG. However, p67-DG activity could not be detected because these cells do not contain a detectable level of p67. The baculovirus expression vector system (BEVS), however, promotes significant expression of cloned p67-cDNA. The expression of p67 was significantly enhanced by the addition of hemin to the growth medium. Maximum enhancement was observed at 5 microM hemin. Data suggest that hemin prevents the activation of latent p67-DG inside the cell and does not have any effect on p67 gene transcription. To gain a better understanding of the mechanism of p67-DG activation and hemin stimulation of p67 synthesis, we have now purified p67-DG from baculovirus-infected insect cells. We prepared antibodies against this protein. These antibodies reacted with a 105-kDa protein in cell extracts from the uninfected insect cells (Sf9), KRC-7, and L929 (animal cells). In addition, these antibodies reacted with an additional 60-kDa protein in the cell extracts of baculovirus-infected Sf9 cells and vaccinia viru...Continue Reading

References

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Jun 15, 1997·Archives of Biochemistry and Biophysics·A BoseN K Gupta

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Citations

May 4, 2011·World Journal of Biological Chemistry·Shiyong Wu
Aug 26, 1998·Biochemical and Biophysical Research Communications·M ChatterjeeN K Gupta
Oct 31, 2000·Journal of the Neurological Sciences·B C WhiteG S Krause
Sep 1, 2009·Biochimica Et Biophysica Acta·Bansidhar Datta

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