Jul 14, 2010

Viral load drives disease in humans experimentally infected with respiratory syncytial virus

American Journal of Respiratory and Critical Care Medicine
John P DevincenzoRobert Lambkin-Williams

Abstract

Respiratory syncytial virus (RSV) is the leading cause of childhood lower respiratory infection, yet viable therapies are lacking. Two major challenges have stalled antiviral development: ethical difficulties in performing pediatric proof-of-concept studies and the prevailing concept that the disease is immune-mediated rather than being driven by viral load. The development of a human experimental wild-type RSV infection model to address these challenges. Healthy volunteers (n = 35), in five cohorts, received increasing quantities (3.0-5.4 log plaque-forming units/person) of wild-type RSV-A intranasally. Overall, 77% of volunteers consistently shed virus. Infection rate, viral loads, disease severity, and safety were similar between cohorts and were unrelated to quantity of RSV received. Symptoms began near the time of initial viral detection, peaked in severity near when viral load peaked, and subsided as viral loads (measured by real-time polymerase chain reaction) slowly declined. Viral loads correlated significantly with intranasal proinflammatory cytokine concentrations (IL-6 and IL-8). Increased viral load correlated consistently with increases in multiple different disease measurements (symptoms, physical examination, an...Continue Reading

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Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Respiratory Syncytial Virus Infections
Antivirals, topical
Virus Titer
Bacteriophage Plaque Assay
Dental Plaque
Chemokine
Respiratory Tract Infections
Senile Plaques
Lower Respiratory Tract Infection

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