Viral load, organ distribution, histopathological lesions, and cytokine mRNA expression in goats infected with a molecular clone of the caprine arthritis encephalitis virus

Virology
A P RavazzoloGiuseppe Bertoni

Abstract

Caprine arthritis encephalitis virus (CAEV) is a lentivirus of goats that causes persistent infection characterized by the appearance of inflammatory lesions in various organs. To define the sites of persistence, 5 goats were infected with a molecular clone of CAEV, and the viral load was monitored by real-time-PCR and RT-PCR in different sites 8 years after infection. The lymph nodes proved to be an important virus reservoir, with moderate virus replication relative to what is reported for lentiviruses of primates. Mammary gland and milk cells were preferred sites of viral replication. The viral load varied significantly between animals, which points to an important role of the genetic background. We found a clear association between occurrence of histopathological lesions and viral load in specific sites. The mRNA expression analysis of several cytokines did not reveal differences between animals that could explain the considerable individual variations in viral load observed.

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Citations

Aug 26, 2014·Viruses·Nancy StonosNiel Karrow
May 18, 2012·Veterinary Research·Helena CrespoRamsés Reina
Aug 31, 2010·Archives of Virology·A MegaliR Zanoni
Jan 8, 2016·Veterinary Research·Leticia SanjoséRamses Reina
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May 20, 2020·Frontiers in Veterinary Science·Irache EcheverríaRamsés Reina
Jan 1, 2014·Journal of Veterinary Medicine·A DeubelbeissR Zanoni
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Nov 13, 2017·Comparative Immunology, Microbiology and Infectious Diseases·Hallgrímur ArnarsonValgerdur Andrésdóttir
Aug 17, 2021·Comparative Immunology, Microbiology and Infectious Diseases·Juscilânia Furtado AraújoMaria Fátima da Silva Teixeira

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