Viral paratransgenesis in the malaria vector Anopheles gambiae.

PLoS Pathogens
Xiaoxia RenJason L Rasgon

Abstract

Paratransgenesis, the genetic manipulation of insect symbiotic microorganisms, is being considered as a potential method to control vector-borne diseases such as malaria. The feasibility of paratransgenic malaria control has been hampered by the lack of candidate symbiotic microorganisms for the major vector Anopheles gambiae. In other systems, densonucleosis viruses (DNVs) are attractive agents for viral paratransgenesis because they infect important vector insects, can be genetically manipulated and are transmitted to subsequent generations. However, An. gambiae has been shown to be refractory to DNV dissemination. We discovered, cloned and characterized the first known DNV (AgDNV) capable of infection and dissemination in An. gambiae. We developed a flexible AgDNV-based expression vector to express any gene of interest in An. gambiae using a two-plasmid helper-transducer system. To demonstrate proof-of-concept of the viral paratransgenesis strategy, we used this system to transduce expression of an exogenous gene (enhanced green fluorescent protein; EGFP) in An. gambiae mosquitoes. Wild-type and EGFP-transducing AgDNV virions were highly infectious to An. gambiae larvae, disseminated to and expressed EGFP in epidemiologicall...Continue Reading

References

Aug 1, 1995·The Journal of General Virology·S L O'NeillR B Tesh
May 7, 1998·Proceedings. Biological Sciences·W ZhouS O'Neil
Aug 18, 2004·In Vitro Cellular & Developmental Biology. Animal·Erica Suchman, Jonathan Carlson
Aug 18, 2004·In Vitro Cellular & Developmental Biology. Animal·Carmelita G FrondozaLars Lindmark
Sep 24, 2004·Biotechnology and Bioengineering·Rachel SpechtOscar-Werner Reif
Sep 26, 2006·Advances in Virus Research·Jonathan CarlsonLeonid Buchatsky
Oct 10, 2006·Applied and Environmental Microbiology·Jason L RasgonMichael Petridis
Jan 17, 2007·International Journal for Parasitology·Michael A RiehleMarcelo Jacobs-Lorena
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Guido FaviaDaniele Daffonchio

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Citations

Aug 13, 2010·Journal of Virology·Bärbel KaufmannMichael G Rossmann
Sep 3, 2010·The American Journal of Tropical Medicine and Hygiene·Jin-Bao GuXiao-Guang Chen
Sep 9, 2011·Journal of Virology·Tatiana V KapelinskayaDmitry V Mukha
Jun 28, 2013·Journal of Virology·Hanh T PhamPeter Tijssen
Jan 14, 2011·Parasitology Research·Kamaraju RaghavendraAditya P Dash
Jun 5, 2012·Integrative and Comparative Biology·Anbjørg RangbergGro V Amdam
Feb 19, 2016·Malaria Journal·Daniel Gonçalves, Patrick Hunziker
Feb 12, 2013·Trends in Biotechnology·Sibao Wang, Marcelo Jacobs-Lorena
Jul 31, 2012·Journal of Invertebrate Pathology·Guy CaljonJan Van Den Abbeele
Apr 3, 2012·Current Opinion in Microbiology·Irene RicciGuido Favia
Dec 17, 2009·Trends in Parasitology·Peter F Billingsley
Mar 3, 2009·Trends in Biotechnology·Bruno LapiedVincent Corbel
Jan 27, 2009·Trends in Parasitology·Willem Takken, Bart G J Knols
Mar 12, 2016·Parasites & Vectors·Maria Vittoria ManciniGuido Favia
May 31, 2014·Scientific Reports·Yasutsugu SuzukiJason L Rasgon
Jul 30, 2015·Scientific Reports·Yasutsugu SuzukiJason L Rasgon
Jan 9, 2016·Environmental Microbiology Reports·Véronique Apaire-MarchaisBruno Lapied
Oct 13, 2009·Parasitology International·Iliano V Coutinho-AbreuMarcelo Ramalho-Ortigao
Dec 26, 2012·Pathogens and Global Health·Irene RicciGuido Favia
Dec 25, 2012·Journal of Invertebrate Pathology·Katsuhiko ItoKeiko Kadono-Okuda
Apr 27, 2018·Viruses·Ferdinand Nanfack Minkeu, Kenneth D Vernick
Dec 27, 2018·Environmental Microbiology·Mine AltinliMathieu Sicard
Jan 25, 2019·The Journal of Veterinary Medical Science·Yongjin QiuChihiro Sugimoto
May 18, 2012·Proceedings. Biological Sciences·C C MurdockMatthew B Thomas
Sep 13, 2019·Transboundary and Emerging Diseases·Muddassar HameedZhiyong Ma
Dec 7, 2019·Virus Evolution·Mine AltinliMathieu Sicard
Sep 10, 2020·Annual Review of Microbiology·E P CaragataG Dimopoulos
Oct 30, 2012·Toxins·Layla Kamareddine

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Datasets Mentioned

BETA
EU233812

Methods Mentioned

BETA
transgenic
PCR
transfection
transmission electron microscopy
fluorescence microscopy

Software Mentioned

AgDNV
BLAST

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