PMID: 1206798Jan 1, 1975Paper

Viral RNA synthesis and levels of DNA-dependent RNA polymerases during replication of adenovirus 2

Journal of Virology
R WeinmannR G Roeder

Abstract

The rates of RNA synthesis in cultured human KB cells infected by adenovirus 2 were estimated by measuring the endogenous RNA polymerase activities in isolated nuclei. The fungal toxin alpha-amanitin was used to determine the relative and absolute levels of RNA polymerases I, II, and III in nuclei isolated during the course of infection. Whereas the level of endogenous RNA polymerase I activity in nuclei from infected cells remained constant relative to the level in nuclei from mock-infected cells, the endogenous RNA polymerase II and III activities each increased about 10-fold. These increases in endogenous RNA polymerase activities were accompanied by concomitant increases in the rates of synthesis in isolated nuclei of viral mRNA precursor, which was quantitated by electrophoretic analysis on polyacrylamide gels. The cellular RNA polymerase levels were measured with exogenous templates after solubilization and chromatographic resolution of the enzymes on DEAE-Sephadex, using procedures in which no losses of activity were apparent. In contrast to the endogenous RNA polymerase activities in isolated nuclei, the cellular levels of the solubilized class I, II, and III RNA polymerases remained constant throughout the course of th...Continue Reading

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·V E SklarR G Roeder
Jan 1, 1975·Cold Spring Harbor Symposia on Quantitative Biology·L PhilipsonT Persson
Jan 1, 1975·Cold Spring Harbor Symposia on Quantitative Biology·P A SharpS J Flint
Jan 1, 1975·Cold Spring Harbor Symposia on Quantitative Biology·E A CraigH J Raskas
Jan 1, 1975·Cold Spring Harbor Symposia on Quantitative Biology·R WeinmannR G Roeder
Jun 3, 1975·Biochemistry·R AxelG Felsenfield
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·E A Zylber, S Penman
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R Weinmann, R G Roeder
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R WeinmannR G Roeder
Jan 1, 1974·Annual Review of Biochemistry·M J Chamberlin
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J TalH J Raskas
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·U Pettersson, L Philipson
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Mar 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J T ParsonsM Green
Mar 28, 1975·Science·J D Griffith

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Citations

Jan 1, 1978·Archives of Virology·G Campadelli-Fiume
Nov 1, 1982·Journal of Cellular Physiology·M T ShanderR Weinmann
Jan 1, 1979·Pharmacology & Therapeutics. Part B: General & Systematic Pharmacology·S Kit
Jun 1, 1979·Journal of Virology·J T PattonR C Bates
Nov 1, 1979·Journal of Virology·V W Yang, S J Flint
Jun 1, 1981·Molecular and Cellular Endocrinology·K S KatulaS Sridhara
Apr 1, 1983·Molecular and Cellular Biology·C L Castiglia, S J Flint

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