Viral Small-RNA Analysis of Bombyx mori Larval Midgut during Persistent and Pathogenic Cytoplasmic Polyhedrosis Virus Infection

Journal of Virology
Aris ZografidisLuc Swevers

Abstract

The lepidopteran innate immune response against RNA viruses remains poorly understood, while in other insects several studies have highlighted an essential role for the exo-RNAi pathway in combating viral infection. Here, by using deep-sequencing technology for viral small-RNA (vsRNA) assessment, we provide evidence that exo-RNAi is operative in the silkworm Bombyx mori against both persistent and pathogenic infection of B. mori cytoplasmic polyhedrosis virus (BmCPV) which is characterized by a segmented double-stranded RNA (dsRNA) genome. Further, we show that Dicer-2 predominantly targets viral dsRNA and produces 20-nucleotide (nt) vsRNAs, whereas an additional pathway is responsive to viral mRNA derived from segment 10. Importantly, vsRNA distributions, which define specific hot and cold spot profiles for each viral segment, to a considerable degree overlap between Dicer-2-related (19 to 21 nt) and Dicer-2-unrelated vsRNAs, suggesting a common origin for these profiles. We found a degenerate motif significantly enriched at the cut sites of vsRNAs of various lengths which link an unknown RNase to the origins of vsRNAs biogenesis and distribution. Accordingly, the indicated RNase activity may be an important early factor for t...Continue Reading

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Apr 6, 2016·Wiley Interdisciplinary Reviews. RNA·Mei-Ling LiGary Brewer
May 28, 2016·Current Opinion in Microbiology·João T Marques, Jean-Luc Imler
Oct 12, 2018·Nature Reviews. Immunology·Zhongxin GuoShou-Wei Ding
Sep 11, 2019·Archives of Insect Biochemistry and Physiology·Luc SweversJingchen Sun
Jul 6, 2020·Archives of Insect Biochemistry and Physiology·Taohong LiLei Wang
May 6, 2020·Insect Science·Min FengJing-Chen Sun
Jan 29, 2019·Frontiers in Physiology·Elise VogelJozef Vanden Broeck
Jun 12, 2019·Wiley Interdisciplinary Reviews. RNA·Anna KolliopoulouLuc Swevers
Dec 12, 2020·RNA Biology·Fabricio Barbosa Monteiro ArraesMaria Fatima Grossi-de-Sa
Mar 2, 2021·Frontiers in Immunology·Liang Jiang
May 25, 2021·Frontiers in Physiology·Mian Muhammad AwaisJingchen Sun

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