Viral Vector-Based Evaluation of Regulatory Regions in the Neuron-Specific Enolase (NSE) Promoter in Mouse Cerebellum In Vivo

The Cerebellum
Yoichiro ShinoharaHirokazu Hirai

Abstract

We investigated the neuron-specific enolase (NSE) promoter in terms of its promoter strength and neuronal specificity in the cerebellum in vivo. The 1.8 kb rat NSE promoter was divided into three regions, A (0.8 kb), B (0.7 kb), and C (0.3 kb), starting from the 5' side. Then, we made various deletion constructs and assessed them by virally expressing GFP under the control of one of the deleted promoters. Removing region A reduced GFP expression to ~6% of that of the original 1.8 kb promoter. Further deletion of region B (presence of region C alone) did not influence the promoter strength, but removing region B from the original 1.8 kb promoter reduced the GFP expression to ~6% of the original level, similar to the level observed after deletion of region A. Immunohistochemistry showed robust GFP expression in Purkinje cells and modest expression in interneurons by the original promoter. Removing region A and/or region B abolished the GFP expression in Purkinje cells in most cerebellar lobules, with the expression in interneurons almost unchanged. These results suggest that region C, which is a proximal 0.3 kb sequence, contains cis-acting elements that drive transcription predominantly in interneurons. The addition of either re...Continue Reading

References

Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K SakimuraY Takahashi
Jan 1, 1995·Brain Research. Molecular Brain Research·K SakimuraY Takahashi
Nov 1, 2003·Journal of Molecular Neuroscience : MN·Yossef S LevyDaniel Offen
Feb 19, 2009·Journal of Neurochemistry·Fiona E ParkinsonMary Lynn Duckworth
Nov 6, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiaodong BaoElias K Michaelis
Jan 8, 2013·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Koichi MiyakeYukihiko Hirai
Aug 26, 2016·Current Opinion in Virology·Daniel Blessing, Nicole Déglon
Aug 30, 2016·PloS One·Yoichiro ShinoharaHirokazu Hirai

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