Abstract
We investigated the neuron-specific enolase (NSE) promoter in terms of its promoter strength and neuronal specificity in the cerebellum in vivo. The 1.8 kb rat NSE promoter was divided into three regions, A (0.8 kb), B (0.7 kb), and C (0.3 kb), starting from the 5' side. Then, we made various deletion constructs and assessed them by virally expressing GFP under the control of one of the deleted promoters. Removing region A reduced GFP expression to ~6% of that of the original 1.8 kb promoter. Further deletion of region B (presence of region C alone) did not influence the promoter strength, but removing region B from the original 1.8 kb promoter reduced the GFP expression to ~6% of the original level, similar to the level observed after deletion of region A. Immunohistochemistry showed robust GFP expression in Purkinje cells and modest expression in interneurons by the original promoter. Removing region A and/or region B abolished the GFP expression in Purkinje cells in most cerebellar lobules, with the expression in interneurons almost unchanged. These results suggest that region C, which is a proximal 0.3 kb sequence, contains cis-acting elements that drive transcription predominantly in interneurons. The addition of either re...Continue Reading
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Citations
Feb 5, 2019·CNS & Neurological Disorders Drug Targets·Jiachun XuYan Li
Oct 7, 2018·Molecular Neurobiology·Yoichiro ShinoharaHirokazu Hirai
Aug 19, 2020·Journal of Neuroscience Methods·Ayumu Konno, Hirokazu Hirai
Apr 12, 2020·Neuroscience·Hiroyuki YasuiHirokazu Hirai
May 15, 2021·Neuroscience Letters·Putri T RadhiyantiHirokazu Hirai