Viral vector mimicking and nucleus targeted nanoparticles based on dexamethasone polyethylenimine nanoliposomes: Preparation and evaluation of transfection efficiency

Colloids and Surfaces. B, Biointerfaces
Bizhan Malaekeh-NikoueiReza Kazemi Oskuee

Abstract

Non-viral vectors such as polymers and liposomes have been used as gene delivery systems to overcome intrinsic problems of viral vectors, but transfection efficiency of these vectors is lower than viral vectors. In the present study, we tried to design non-viral gene delivery vectors that mimic the viral vectors using the benefits of both cationic liposomes and cationic polymer vectors along with targeting glucocorticoid receptors to enhance cellular trafficking of vectors. Cationic liposomes containing DOTAP and cholesterol were prepared by thin-film hydration following extrusion method. Dexamethasone mesylate was synthesized and then conjugated to polyethylenimine through a one-step reaction. A novel gene delivery system, Lipopolyplex was developed by premixing liposome and different molecular weight of bPEI-Dexa as carriers followed by addition of plasmid at three different carrier/pDNA (C/P) weight ratios. The resulted complexes were characterized for their size, zeta potential and ability of DNA condensation. Transfection efficiency of vectors in neuro2A was determined by Luciferase reporter gene assay. Also, the toxicity of gene carriers was investigated in this cell line. Mean particle size of prepared complexes was less...Continue Reading

Citations

Dec 10, 2019·Biotechnology and Bioengineering·Zhenpeng LiFang Bai
Jul 3, 2020·Chemical & Pharmaceutical Bulletin·Shintaro Fumoto, Koyo Nishida
Oct 8, 2020·Biomaterials Science·Kübra Kaygisiz, Christopher V Synatschke
Apr 4, 2021·Biomedicines·Natallia V DubashynskayaYury A Skorik

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