Viral Vectors for Gene Delivery and Expression in the CNS

Methods : a Companion to Methods in Enzymology
M G Kaplitt, D W Pfaff

Abstract

Viral vectors have emerged as an important tool for manipulating gene expression in the adult mammalian brain. The adult brain is composed largely of nondividing cells, and therefore DNA viruses have become the vehicle of choice for neurobiologists interested in somatic gene transfer. Recombinant viral vectors based upon adenovirus or herpes simplex virus have been created in which a gene essential for viral replication is removed and a gene of interest is inserted in the viral genome. While this eliminates pathogenicity due to viral replication, retention of viral genes and continued expression of these genes may limit the potential of the current generation of vectors. Defective viral vectors represent a different approach, in which only viral recognition signals are used to allow packaging of foreign DNA into a viral coat while eliminating the possibility of viral gene expression within target cells. The defective HSV vector has been used to transfer genes into the adult rat brain. This vector has also been used for analysis of the preproenkephalin promoter in vivo, and important regions of this promoter have been identified using this technique. A modification of in situ PCR has been developed as an adjunctive tool for sens...Continue Reading

Citations

Jun 24, 1999·The Journal of Veterinary Medical Science·T MurataH Otsuka
Jan 5, 2012·PloS One·Florent BernaudatNorbert Rolland
Mar 27, 2007·Pharmaceutical Research·Véronique RivestFrédéric Calon
Mar 11, 2000·The American Journal of Clinical Hypnosis·E L Rossi

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