PMID: 11607242Dec 1, 1991Paper

virF, the host-range-determining virulence gene of Agrobacterium tumefaciens, affects T-DNA transfer to Zea mays

Proceedings of the National Academy of Sciences of the United States of America
E JarchowB Hohn

Abstract

The monocotyledonous plant Zea mays does not develop tumors after inoculation with Agrobacterium tumefaciens and is thus defined as nonhost. Agroinfection, Agrobacterium-mediated delivery of maize streak virus, demonstrates that transferred DNA (T-DNA) transfer to the plant does occur. Nopaline-type Agrobacterium strains such as C58 are efficient in the transfer process whereas the octopine-type strain A6 is unable to transfer T-DNA to maize. This phenotypic difference maps to the tumor-inducing (Ti) plasmid but not to the T-DNA. Steps preceding T-DNA transfer, such as attachment and induction of the virulence genes, were shown to take place in the octopine strain. The nopaline-plasmid-specific locus tzs and the octopine-plasmid-specific locus pinF (virH) are not involved in the strain specificity. However, mutations in the virF locus rendered the octopine strain agroinfectious on maize, whereas such virF-defective octopine strains, when complemented by virF on a plasmid, completely lost their agroinfectivity. We propose that VirF, known to increase the host range of the bacteria in other systems, acts as an inhibitor of T-DNA transfer to maize.

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Citations

Dec 1, 1992·Plant Molecular Biology·X Q LiT K Hodges
Jul 1, 1996·World Journal of Microbiology & Biotechnology·K Weising, G Kahl
Aug 12, 2006·Canadian Journal of Physiology and Pharmacology·Benoît LacroixVitaly Citovsky
Oct 22, 2008·Yi chuan = Hereditas·Su-Zhi Zhang, Ting-Zhao Rong
Jul 24, 2014·Molecular Plant-microbe Interactions : MPMI·Arsheed Hussain SheikhAlok Krishna Sinha
Jun 10, 2009·Seminars in Cell & Developmental Biology·Vitaly CitovskyAlexander Krichevsky
Nov 21, 2015·Scientific Reports·Benoît Lacroix, Vitaly Citovsky
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Stanton B Gelvin
May 29, 2008·Journal of Basic Microbiology·Ismail Saadoun, Fouad Al-Momani
Dec 9, 2020·Biotechnology Advances·Jonas De SaegerStephen Depuydt

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