Viridans Group Streptococci clinical isolates: MALDI-TOF mass spectrometry versus gene sequence-based identification

PloS One
Silvia AngelettiLucia De Florio

Abstract

Viridans Group Streptococci (VGS) species-level identification is fundamental for patients management. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been used for VGS identification but discrimination within the Mitis group resulted difficult. In this study, VGS identifications with two MALDI-TOF instruments, the Biotyper (Bruker) and the VITEK MS (bioMérieux) have been compared to those derived from tuf, soda and rpoB genes sequencing. VGS isolates were clustered and a dendrogram constructed using the Biotyper 3.0 software (Bruker). RpoB gene sequencing resulted the most sensitive and specific molecular method for S. pneumonia identification and was used as reference method. The sensitivity and the specificity of the VITEK MS in S. pneumonia identification were 100%, while the Biotyper resulted less specific (92.4%). In non pneumococcal VGS strains, the group-level correlation between rpoB and the Biotyper was 100%, while the species-level correlation was 61% after database upgrading (than 37% before upgrading). The group-level correlation between rpoB and the VITEK MS was 100%, while the species-level correlation was 36% and increases at 69% if isolates identified as S. mitis/...Continue Reading

References

Aug 30, 2001·Journal of Clinical Microbiology·J A KelloggM C Smith
Oct 5, 2002·Clinical Microbiology Reviews·Richard Facklam
Aug 7, 2004·Journal of Clinical Microbiology·François J PicardMichel G Bergeron
Aug 18, 2004·Diagnostic Microbiology and Infectious Disease·Trudy O MessmerRichard R Facklam
Jun 1, 2007·Journal of Clinical Microbiology·Maria da Gloria S CarvalhoJacquelyn S Sampson
Jun 8, 2007·Journal of Clinical Microbiology·C FriedrichsK Eschrich
Sep 16, 2008·International Journal of Antimicrobial Agents·Jae-Hoon SongKwan Soo Ko
Aug 24, 2010·International Journal of Medical Microbiology : IJMM·Manuel WoltersMartin Christner
Sep 3, 2010·Journal of Clinical Microbiology·Christopher D Doern, Carey-Ann D Burnham
Oct 29, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C TelesS Lang
Apr 19, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P López RoaE Bouza
Nov 10, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·P KärpänojaH Sarkkinen
Dec 24, 2013·F1000Research·Cristiane C ThompsonAna Carolina P Vicente
Apr 23, 2014·Emerging Infectious Diseases·Samuel A ShelburneAnthony R Flores
Jun 6, 2014·Journal of Clinical Microbiology·Jessica Galloway-PeñaSamuel A Shelburne

❮ Previous
Next ❯

Citations

Mar 15, 2016·Diagnostic Microbiology and Infectious Disease·Joffrey van PrehnEls Wessels
Mar 19, 2016·Travel Medicine and Infectious Disease·Silvia AngelettiUNKNOWN Sanitary Bureau of Asylum Seekers Center of Castelnuovo di Porto
May 26, 2016·Diagnostic Microbiology and Infectious Disease·Daniel TenaJuan Antonio Sáez-Nieto
Jan 25, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W-T FanB Gu
Aug 25, 2020·Circulation·Christopher J AllenBernard D Prendergast
Sep 22, 2020·Future Microbiology·Michał ZłochBogusław Buszewski
Dec 4, 2019·Antimicrobial Resistance and Infection Control·Maja Kosecka-StrojekAlexander W Friedrich
Jul 12, 2017·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Marta Kłos, Jadwiga Wójkowska-Mach
May 31, 2019·Case Reports in Infectious Diseases·Lyra B OlsonChristopher J Hostler
Aug 30, 2018·Frontiers in Microbiology·Lucia De FlorioSilvia Angeletti
Nov 20, 2020·Journal of Clinical Microbiology·Christian Salgård JensenXiaohui Chen Nielsen
Nov 19, 2020·European Heart Journal. Case Reports·James S TomlinsonRachael James
Apr 3, 2021·Frontiers in Cellular and Infection Microbiology·Aline CuénodAdrian Egli
Jul 7, 2017·Journal of the American Society for Mass Spectrometry·Klaudia I KocurekHelen J Cooper
Feb 19, 2021·Journal of Clinical Microbiology·Linoj P SamuelUNKNOWN ASM Clinical and Public Health Microbiology Committee
Aug 24, 2021·Frontiers in Cellular and Infection Microbiology·Mohamed M H AbdelbaryGeorg Conrads

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

VITEK
VITEK Compact
ClinproTool
Bruker Biotyper
ClinproTools
FlexControl
Biotyper
MALDI Biotyper

Related Concepts

Related Feeds

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Meningitis (ASM)

Bacterial meningitis continues to be an important cause of mortality and morbidity throughout the world. Here is the latest research.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Meningitis

Bacterial meningitis continues to be an important cause of mortality and morbidity throughout the world. Here is the latest research.