Virion-associated HIV-1 Vpr: variable amount in virus particles derived from cells upon virus infection or proviral DNA transfection

Virology
S P SinghA Srinivasan

Abstract

Human immunodeficiency virus type-1 (HIV-1) Vpr is a virion-associated protein implicated to have a role in AIDS pathogenesis. In regard to the amount of Vpr incorporated into virus particles, the published data vary widely. To address this, we quantitated Vpr in virus particles derived from diverse sources that are used to evaluate the biological effect of Vpr. Virus particles from infected cells showed only a small amount of Vpr. Interestingly, virus particles from cells cotransfected with HIV-1 proviral DNA lacking Vpr coding sequences (NLDeltaVpr) and a Vpr expression plasmid showed a drastic increase (29.4-fold) in the incorporation of Vpr. Furthermore, cotransfection involving NLDeltaVpr and different concentrations of Vpr expression plasmid resulted in virus particles containing Vpr in proportion to the Vpr expression plasmid used. The differences in virus particles with respect to Vpr as revealed by these studies should be taken into account in assessing the effect of Vpr.

References

Jun 1, 1990·Journal of Virology·E A CohenW A Haseltine
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·S MahalingamA Srinivasan
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·D N LevyD B Weiner
Jan 8, 1999·Journal of Molecular Biology·B G Turner, M F Summers
Jun 18, 1999·Reviews in Medical Virology·M Bukrinsky, A Adzhubei

❮ Previous
Next ❯

Citations

May 15, 2004·DNA and Cell Biology·Parithosh K TungaturthiAlagarsamy Srinivasan
Mar 11, 2009·AIDS Research and Human Retroviruses·Bizhan RomaniSusan Engelbrecht
Nov 20, 2009·Journal of Virology·Joëlle V FritzHugues de Rocquigny
Mar 19, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Max W RichardsonJay Rappaport
Nov 6, 2002·The Journal of Cell Biology·David McDonaldThomas J Hope
Jun 7, 2016·Reviews in Medical Virology·Rui SoaresTeresa Gonçalves
Jun 28, 2016·Sensors·Daniel M Jones, Sergi Padilla-Parra
Aug 13, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Silvia PerettiMaurizio Federico
Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Joanna BednarskaAndrew Shevchuk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.