Virome Identification and Characterization of Fusarium sacchari and F. andiyazi : Causative Agents of Pokkah Boeng Disease in Sugarcane

Frontiers in Microbiology
Ziting YaoMuqing Zhang

Abstract

Fusarium sacchari and Fusarium andiyazi are two devastating sugarcane pathogens that cause pokkah boeng disease (PBD) in China. RNA_Seq was conducted to identify mycoviruses in F. sacchari and F. andiyazi isolates collected from PBD symptom-showing sugarcane plants across China. Fifteen isolates with a normal, debilitated, or abnormal phenotype in colony morphology were screened out for the existence of dsRNA from 104 Fusarium isolates. By sequencing the mixed pool of dsRNA from these Fusarium isolates, a total of 26 contigs representing complete or partial genome sequences of ten mycoviruses and their strains were identified, including one virus belonging to Hypoviridae, two mitoviruses with seven strains belonging to Narnaviridae, one virus of Chrysoviridae, and one alphavirus-like virus. RT-PCR amplification with primers specific to individual mycoviruses revealed that mitoviruses were the most prevalent and the alphavirus-like virus and chrysovirus were the least prevalent. In terms of host preference, more mitoviruses were found in F. andiyazi than in F. sacchari. Fusarium sacchari hypovirus 1 with a 13.9 kb genome and a defective genome of 12.2 kb, shares 54% identity at the amino acid level to the Wuhan insect virus 14, ...Continue Reading

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Datasets Mentioned

BETA
MN295967

Methods Mentioned

BETA
electrophoresis
Illumina sequencing
RNAseq
transmission electron microscopy
transgenic

Software Mentioned

BLAST
BLASTn
Trinity RNAseq
BLASTx
Trinity
MEGA
Blastp

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