Virtual-freezing fluorescence imaging flow cytometry.

Nature Communications
Hideharu MikamiKeisuke Goda

Abstract

By virtue of the combined merits of flow cytometry and fluorescence microscopy, imaging flow cytometry (IFC) has become an established tool for cell analysis in diverse biomedical fields such as cancer biology, microbiology, immunology, hematology, and stem cell biology. However, the performance and utility of IFC are severely limited by the fundamental trade-off between throughput, sensitivity, and spatial resolution. Here we present an optomechanical imaging method that overcomes the trade-off by virtually freezing the motion of flowing cells on the image sensor to effectively achieve 1000 times longer exposure time for microscopy-grade fluorescence image acquisition. Consequently, it enables high-throughput IFC of single cells at >10,000 cells s-1 without sacrificing sensitivity and spatial resolution. The availability of numerous information-rich fluorescence cell images allows high-dimensional statistical analysis and accurate classification with deep learning, as evidenced by our demonstration of unique applications in hematology and microbiology.

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Citations

Jul 10, 2020·Lab on a Chip·Akihiro IsozakiKeisuke Goda
May 28, 2020·Lab on a Chip·Akihiro IsozakiKeisuke Goda
Dec 17, 2020·Lab on a Chip·Biagio MandracchiaShu Jia
Feb 6, 2021·Bioanalysis·Sonal M ManoharAnusree Nair
Oct 30, 2020·Optics Express·Yueyun WengCheng Lei
Jan 30, 2021·Trends in Biotechnology·Yuqi ZhouKeisuke Goda
Apr 1, 2021·Accounts of Chemical Research·Julia Gala de PabloKeisuke Goda
May 20, 2021·NPJ Systems Biology and Applications·Matthew A RodriguesVidya Venkatachalam
May 26, 2021·Cytometry. Part B, Clinical Cytometry·Jason StanleyKathy Fuller
Jun 15, 2021·Lab on a Chip·Erick J Vargas-OrdazVictor J Cadarso
Aug 12, 2021·Biosensors & Bioelectronics·Tao TangYaxiaer Yalikun
Sep 14, 2021·Lab on a Chip·Andreas KleiberWolfgang Fritzsche

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Methods Mentioned

BETA
flow cytometry
fluorescence
fluorescence imaging
fluorescence microscopy
flow
environmental stress
electron microscopy
chip
PCR
FACS

Software Mentioned

Python
CV
Numpy
PCO Camware
scikit
image
LabVIEW
sim
Scipy
Keras

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