Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus

Parasites & Vectors
Melbourne Rio TalactacMasami Mochizuki

Abstract

Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis. Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat virus (LGTV), a tick-borne flavivirus. Longicin P1 and P4 peptides were chemically synthesized. Antiviral activity of the longicin peptides against LGTV was evaluated through in vitro virucidal assays, wherein the antiviral efficacy was determined by reduction in number of viral foci and virus yield. Additionally, longicin P4 was also tested for its activity against human adenovirus, a non-enveloped virus. Lastly, to assess the importance of longicin on the innate antiviral immunity of H. longicornis ticks, gene silencing through RNAi was performed. Longicin P4 produced significant viral foci reduction and lower virus yield against LGTV, while longicin P1 failed to demonstrate the same results. Conversely, both longicin partial analogs (P1 and P4) did not show significant antiviral activity when tested on adenovirus. In addition, longicin-silenced ticks showed significa...Continue Reading

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Citations

Dec 20, 2018·Frontiers in Physiology·Melbourne Rio TalactacTetsuya Tanaka
Aug 12, 2017·Frontiers in Cellular and Infection Microbiology·Mária KazimírováPatricia A Nuttall
Feb 13, 2020·Frontiers in Cellular and Infection Microbiology·Kodai KusakisakoTetsuya Tanaka
Jan 24, 2021·Developmental and Comparative Immunology·Melbourne Rio TalactacTetsuya Tanaka

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Methods Mentioned

BETA
Assay
PCR
in vitro transcription
electrophoresis
Fluorescence

Software Mentioned

Quantity One
7300 system SDS
GraphPad Prism

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