Abstract
Longicin is a defensin-like peptide, identified from the midgut epithelium of hard tick Haemaphysalis longicornis. Several studies have already shown the antimicrobial and parasiticidal activities of longicin peptide and one of its synthetic partial analogs, longicin P4. In this study, longicin peptides were tested for potential antiviral activity against Langat virus (LGTV), a tick-borne flavivirus. Longicin P1 and P4 peptides were chemically synthesized. Antiviral activity of the longicin peptides against LGTV was evaluated through in vitro virucidal assays, wherein the antiviral efficacy was determined by reduction in number of viral foci and virus yield. Additionally, longicin P4 was also tested for its activity against human adenovirus, a non-enveloped virus. Lastly, to assess the importance of longicin on the innate antiviral immunity of H. longicornis ticks, gene silencing through RNAi was performed. Longicin P4 produced significant viral foci reduction and lower virus yield against LGTV, while longicin P1 failed to demonstrate the same results. Conversely, both longicin partial analogs (P1 and P4) did not show significant antiviral activity when tested on adenovirus. In addition, longicin-silenced ticks showed significa...Continue Reading
References
Jul 30, 1999·Developmental and Comparative Immunology·P BuletD Hoffmann
Oct 16, 1999·Science·Y Q TangM E Selsted
Sep 16, 2000·Trends in Microbiology·R E Hancock, G Diamond
Jan 25, 2002·Nature·Michael Zasloff
Oct 23, 2003·Experimental & Applied Acarology·Yoshiro NakajimaMinoru Yamakawa
Dec 24, 2003·Advances in Virus Research·P A Nuttall, M Labuda
Nov 24, 2004·International Journal of Antimicrobial Agents·K V R ReddyC Aranha
Jun 9, 2005·Parasitology·F Jongejan, G Uilenberg
Oct 11, 2005·Biotechnology Letters·Kris De Smet, Roland Contreras
Oct 18, 2005·Peptides·Haiqin ChenPeilin Cen
May 9, 2007·Infection and Immunity·Naotoshi TsujiKozo Fujisaki
Jan 15, 2008·Cell Host & Microbe·Jason G Smith, Glen R Nemerow
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Jose de la FuenteDaniel E Sonenshine
Jun 3, 2008·Lancet·Lars Lindquist, Olli Vapalahti
Jul 29, 2008·Molecular Immunology·Caihuan TianShunyi Zhu
Jun 23, 2009·Virology·Shanna L AshleyKatherine R Spindler
Jul 21, 2009·Biochemical and Biophysical Research Communications·Bin GaoShunyi Zhu
Nov 27, 2009·The Journal of Veterinary Medical Science·Morshedur RahmanKozo Fujisaki
May 17, 2011·Cellular and Molecular Life Sciences : CMLS·Amy T Y YeungRobert E W Hancock
Aug 19, 2011·Peptides·Tetsuya TanakaKozo Fujisaki
Jan 10, 2012·Virology Journal·Sharon E AltmannJoseph E Blaney
Nov 13, 2012·BMC Complementary and Alternative Medicine·Keivan ZandiSazaly Abubakar
Feb 9, 2013·The Journal of Experimental Biology·Remil Linggatong GalayTetsuya Tanaka
Jul 23, 2013·Frontiers in Cellular and Infection Microbiology·Ondřej HajdušekPetr Kopáček
Nov 10, 2013·Cancers·Yuma YamadaHideyoshi Harashima
Nov 16, 2013·Nature Chemical Biology·Ashley L HilchieRobert E W Hancock
Aug 15, 2014·Journal of Virology·Elvira WeberAndrea Kröger
Dec 3, 2014·Parasites & Vectors·Miray TonkMohammad Rahnamaeian
Jan 3, 2016·Developmental and Comparative Immunology·Hiroki MaedaTetsuya Tanaka
Citations
Dec 20, 2018·Frontiers in Physiology·Melbourne Rio TalactacTetsuya Tanaka
Aug 12, 2017·Frontiers in Cellular and Infection Microbiology·Mária KazimírováPatricia A Nuttall
Feb 13, 2020·Frontiers in Cellular and Infection Microbiology·Kodai KusakisakoTetsuya Tanaka
Jan 24, 2021·Developmental and Comparative Immunology·Melbourne Rio TalactacTetsuya Tanaka
Nov 27, 2020·Acta Tropica·Emmanuel Pacia HernandezTetsuya Tanaka
Nov 23, 2016·Developmental and Comparative Immunology·Melbourne Rio TalactacTetsuya Tanaka
Jun 3, 2021·Viruses·Miray TonkAndreas Vilcinskas