Virulence-defective strains of Salmonella enterica serovar Typhi as candidates for education at level 2 facilities

Microbiology and Immunology
Xinxiang HuangT Ezaki

Abstract

The use of biosafety level 3 pathogens is an essential element of education and training at medical schools. We previously reported on invasion-defective strains of Salmonella enterica serovar Typhi, GTC 3P408 (DeltainvA, DeltasipB) and GTC 3P409 (DeltainvA, DeltasipB, and DeltaviaB), as candidates for use in educational programs. Vi negative strains of S. enterica serovar Typhi became extremely sensitive to complement attack but showed increased invasiveness. Therefore, this study was conducted to construct two virulencedefective strains, GTC 3P460 (DeltainvA, DeltasipB, and DeltarpoS) and GTC 3P461 (DeltainvA, DeltasipB, DeltaviaB, and DeltarpoS), of S. enterica serovar Typhi by deleting rpoS from the GTC 3P409 and GTC 3P408 strains. Stress tests demonstrated that GTC 3P460 and GTC 3P461 are sensitive to conditions of starvation, acid stress and oxidative stress. These results suggest that these virulence-defective strains have difficulty surviving in the gastric environment and in macrophages, characteristics that make them ideal candidates for education at level 2 facilities. Colony morphology and conventional biochemical features of these strains are identical to the parent strain S. enterica serovar Typhi GIFU 10007.

References

Sep 1, 1984·The Journal of Infectious Diseases·J D Robbins, J B Robbins
Jul 1, 1995·Nihon saikingaku zasshi. Japanese journal of bacteriology·T Ezaki, H Watanabe
Sep 21, 2000·Pathophysiology : the Official Journal of the International Society for Pathophysiology·K Kannan, S K Jain
Dec 8, 2000·Advances in Experimental Medicine and Biology·R Hengge-Aronis
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Regine Hengge-Aronis
Apr 20, 2004·Environmental Pollution·M Khan, J Scullion
May 12, 2004·FEMS Microbiology Letters·Xinxiang HuangTakayuki Ezaki

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Citations

Feb 19, 2014·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Xiumei ShengXinxiang Huang

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