Virulence properties and serotypes of Shiga toxin-producing Escherichia coli from healthy Australian slaughter-age sheep

Journal of Clinical Microbiology
S P DjordjevicK A Bettelheim

Abstract

A group of 1,623 ovine fecal samples recovered from 65 geographically distinct mutton sheep and prime lamb properties across New South Wales, Australia, were screened for the presence of Shiga toxin-producing Escherichia coli (STEC) virulence factors (stx(1), stx(2), eaeA, and ehxA). A subset was cultured for STEC isolates containing associated virulence factors (eaeA and/or ehxA), which were isolated from 17 of 20 (85%) and 19 of 20 (95%) tested prime lamb and mutton sheep properties, respectively. STEC isolates containing stx(1), stx(2), and ehxA were most commonly isolated (19 of 40 flocks; 47.5%), and this profile was observed for 10 different serotypes. Among 90 STEC isolates studied, the most common serotypes were O91:H(-) (22 isolates [24.4%]), O5:H(-) (16 isolates [17.8%]), O128:H2 (11 isolates [12.2%]), O123:H(-) (8 isolates [8.9%]), and O85:H49 (5 isolates [5.6%]). Two isolates (2.2%) were typed as O157:H(-). A total of 78 of 90 STEC isolates (86.7%) expressed Shiga toxin in Vero cell culture and 75 of 84 ehxA-positive isolates (89.3%) expressed enterohemolysin on washed sheep blood agar. eaeA was observed in 11 of 90 (12.2%) ovine STEC isolates, including serotypes O5:H(-), O84:H(-), O85:H49, O123:H(-) O136:H40, and ...Continue Reading

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Citations

Apr 22, 2011·Applied Biochemistry and Biotechnology·Mohamed BennaniHamid Amarouch
Jul 2, 2010·Foodborne Pathogens and Disease·Robert Steven Barlow, Glen Ean Mellor
Apr 12, 2012·Applied and Environmental Microbiology·Lin T BrandalGeorg Kapperud
Oct 18, 2011·Applied and Environmental Microbiology·Aine MonaghanDeclan J Bolton
Mar 27, 2012·Applied and Environmental Microbiology·Anna-Katarina SchillingLutz Geue
Nov 27, 2002·Applied and Environmental Microbiology·Michael A HornitzkySteven P Djordjevic
Nov 8, 2003·Journal of Clinical Microbiology·Vidiya RamachandranSteven P Djordjevic
Aug 9, 2003·Journal of Clinical Microbiology·Kari S GobiusPatricia M Desmarchelier
Jul 9, 2004·International Journal of Food Microbiology·C ZweifelR Stephan
Jun 24, 2006·International Journal of Food Microbiology·Robert S BarlowPatricia M Desmarchelier
Feb 12, 2009·FEMS Microbiology Reviews·Roberto M La RagioneAndrew D Wales
Apr 25, 2007·Critical Reviews in Microbiology·Karl A Bettelheim
Apr 3, 2003·Experimental Biology and Medicine·Karl A Bettelheim
Sep 7, 2007·Journal of Food Protection·Norasak KalchayanandMohammad Koohmaraie
Jan 5, 2021·Foodborne Pathogens and Disease·Siobhán C McCarthyGeraldine Duffy

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