Virus detection and monitoring of viral load in Crimean-Congo hemorrhagic fever virus patients.

Emerging Infectious Diseases
Roman WölfelChristian Drosten

Abstract

We developed a real-time reverse transcription--PCR that detected 1,164 copies/mL of Crimean-Congo hemorrhagic fever virus per milliliter of serum at 95% probability (probit analysis) and was 100% concordant with nested PCR on 63 samples from 31 patients with confirmed infection. Infected patients who died appeared to have higher viral loads; low viral loads correlated with IgG detection.

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Methods Mentioned

BETA
PCR

Software Mentioned

TreeCon
Primer Express

Related Concepts

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