Virus induced gene silencing of a DEFICIENS ortholog in Nicotiana benthamiana

Plant Molecular Biology
Yule LiuS P Dinesh-Kumar

Abstract

Traditionally, developmental studies in plant biology have suffered from the lack of a convenient means to study gene function in non-model plant species. Here we show that virus-induced gene silencing (VIGS) is an effective new tool to study the function of orthologs of floral homeotic genes such as DEFICIENS (DEF) in non-model systems. We used a tobacco rattle virus (TRV)-based VIGS approach to study the function of the Nicotiana benthamiana DEF ortholog (NbDEF). Silencing of NbDEF in N. benthamiana using TRV-VIGS was similar to that of Antirrhinum def and Arabidopsis ap3 mutants and caused transformation of petals into sepals and stamens into carpels. Molecular analysis of the NbDEF -silenced plants revealed a dramatic reduction of the levels of NbDEF mRNA and protein in flowers. NbDEF silencing was specific and has no effect on the mRNA levels of NbTM6, the closest paralog of NbDEF. A dramatic reduction of the levels of N. benthamiana GLOBOSA (NbGLO) mRNA and protein was also observed in flowers of NbDEF-silenced plants, suggesting that cross-regulation of this GLO-like gene by NbDEF. Taken together, our results suggest that NbDEF is a functional homolog of Antirrhinum DEF. Our results are significant in that they show that...Continue Reading

Citations

Dec 27, 2011·Development Genes and Evolution·Jacob B LandisLena C Hileman
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Solomon StonebloomPatricia Zambryski
May 16, 2009·Journal of Experimental Botany·Vivian F Irish
Jan 6, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Elena M Kramer, Scott A Hodges
Jul 4, 2006·Plant Physiology·Tessa M Burch-SmithS P Dinesh-Kumar
Jan 29, 2013·Plant Science : an International Journal of Experimental Plant Biology·Ming-Hsien HsiehHong-Hwa Chen
Sep 6, 2011·Plant Science : an International Journal of Experimental Plant Biology·Changming ChenJianjun Lei
Mar 15, 2012·Science China. Life Sciences·Changjun HuangXueping Zhou
Sep 29, 2009·Plant Physiology and Biochemistry : PPB·Arunima Purkayastha, Indranil Dasgupta
Aug 19, 2004·The Plant Journal : for Cell and Molecular Biology·Tessa M Burch-SmithS P Dinesh-Kumar
Jul 7, 2005·The Plant Journal : for Cell and Molecular Biology·Da-Qi FuYun-Bo Luo
Nov 14, 2012·The Plant Journal : for Cell and Molecular Biology·Edelín RoqueLuis A Cañas
Jun 29, 2012·Molecular Plant Pathology·Parijat S JuvaleThomas J Baum
Jul 12, 2011·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Verónica S Di Stilio
Jan 25, 2012·Journal of Integrative Plant Biology·Haiping LiuYunbo Luo
Nov 13, 2009·Plant Biotechnology Journal·Jian YeNam-Hai Chua
Feb 17, 2006·Current Opinion in Plant Biology·Jimena Carrillo-TrippRafael Rivera-Bustamante
Jun 21, 2005·Current Opinion in Genetics & Development·Vivian F Irish, Amy Litt
Apr 14, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ana Maria Rocha de AlmeidaChelsea D Specht

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