Virus-Induced Gene Silencing of the Eggplant Chalcone Synthase Gene during Fruit Ripening Modifies Epidermal Cells and Gravitropism

Journal of Agricultural and Food Chemistry
Cuicui Wang, Daqi Fu

Abstract

Eggplant ( Solanum melongena L.) fruits accumulate flavonoids in their cuticle and epidermal cells during ripening. Although many mutants available in model plant species, such as Arabidopsis thaliana and Medicago truncatula, are enabling the intricacies of flavonoid-related physiology to be deduced, the mechanisms whereby flavonoids influence eggplant fruit physiology are unknown. Virus-induced gene silencing (VIGS) is a reliable tool for the study of flavonoid function in fruit, and in this study, we successfully applied this technique to downregulate S. melongena chalcone synthase gene ( SmCHS) expression during eggplant fruit ripening. In addition to the expected change in fruit color attributable to a lack of anthocyanins, several other modifications, including differences in epidermal cell size and shape, were observed in the different sectors. We also found that silencing of CHS gene expression was associated with a negative gravitropic response in eggplant fruits. These observations indicate that epidermal cell expansion during ripening is dependent upon CHS expression and that there may be a relationship between CHS expression and gravitropism during eggplant fruit ripening.

References

Mar 4, 2000·Plant Physiology·A M RashotteG K Muday
Jan 25, 2002·Critical Reviews in Plant Sciences·J Z Kiss
Mar 9, 2002·The Plant Cell·Gloria K Muday, Angus S Murphy
Sep 11, 2002·The Plant Journal : for Cell and Molecular Biology·Yule LiuS P Dinesh-Kumar
Oct 15, 2003·Trends in Plant Science·Gérald Perbal, Dominique Driss-Ecole
Dec 19, 2003·Plant Physiology·Elison B Blancaflor, Patrick H Masson
Jul 30, 2004·Natural Product Reports·Christine A Williams, Renee J Grayer
Apr 30, 2005·Current Opinion in Plant Biology·Loverine P Taylor, Erich Grotewold
Jul 7, 2005·The Plant Journal : for Cell and Molecular Biology·Da-Qi FuYun-Bo Luo
Nov 1, 2005·Yonsei Medical Journal·Joo-Heon Yoon, Seung Joon Baek
Aug 1, 1979·Plant Physiology·R Ben-Arie, N Kislev
May 4, 2006·Annual Review of Plant Biology·Harsh P BaisJorge M Vivanco
Mar 6, 2007·Plant Physiology·Jan Maisch, Peter Nick
Aug 28, 2007·Plant Physiology·Charles S BuerMichael A Djordjevic
Jul 15, 1988·Science·M Jacobs, P H Rubery
Jan 18, 2008·Trends in Plant Science·Wendy Ann Peer, Angus S Murphy
Aug 23, 2008·The Journal of Biological Chemistry·Diana SanteliaEnrico Martinoia
Jan 9, 2009·Journal of Experimental Botany·Charles S Buer, Michael A Djordjevic
Jul 28, 2009·Plant Physiology·Peter NickGyeunhung An
Jun 1, 2008·Plant Signaling & Behavior·Charles S BuerMichael A Djordjevic
Jan 16, 2010·Journal of Integrative Plant Biology·Charles S BuerMichael A Djordjevic
Jul 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Gang LiDabing Zhang
Jun 4, 2015·Plant Signaling & Behavior·Antonio HerediaEva Domínguez
Jan 26, 2016·Journal of Agricultural and Food Chemistry·Karsten BeekmannIvonne Magdalena Catharina Maria Rietjens
Mar 7, 2017·Journal of Agricultural and Food Chemistry·Tian Tian XingYuan Fang Li
Apr 15, 2017·Journal of Agricultural and Food Chemistry·Anna C CreceliusUlrich S Schubert
Nov 22, 2017·Biochemistry Research International·Zübeyir Huyutİlhami Gülçin
Jul 1, 2008·Functional Plant Biology : FPB·Eva DomínguezAntonio Heredia

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