Virus-induced gene silencing (VIGS) for functional genomics in rice using Rice tungro bacilliform virus (RTBV) as a vector

Methods in Molecular Biology
Ravi KantI Dasgupta

Abstract

The large-scale functional analysis of genes in plants depends heavily on robust techniques for gene silencing. Virus-induced gene silencing (VIGS) is a transient gene silencing method for plants, triggered by the inoculation of a modified viral vector carrying a fragment of the gene targeted for silencing. Here we describe a VIGS protocol for rice, based on the Rice tungro bacilliform virus (RTBV, a DNA virus). We present an updated and detailed protocol for silencing of the gene encoding Phytoene desaturase in rice, using the RTBV-VIGS system.

Citations

May 26, 2017·Plant Cell Reports·Ravi Kant, Indranil Dasgupta
Aug 11, 2019·Scientific Reports·Yuh TzeanHsin-Hung Yeh

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