Virus-induced multiple gene silencing to study redundant metabolic pathways in plants: silencing the starch degradation pathway in Nicotiana benthamiana

Biotechnology Journal
Gavin M GeorgeJames R Lloyd

Abstract

Virus-induced gene silencing (VIGS) is a rapid technique that allows for specific and reproducible post-transcriptional degradation of targeted mRNA. The method has been proven efficient for suppression of expression of many single enzymes. The metabolic networks of plants, however, often contain isoenzymes and gene families that are able to compensate for a mutation and mask the development of a silencing phenotype. Here, we show the application of multiple gene VIGS repression for the study of these redundant biological pathways. Several genes in the starch degradation pathway [disproportionating enzyme 1; (DPE1), disproportionating enzyme 2 (DPE2), and GWD] were silenced. The functionally distinct DPE enzymes are present in alternate routes for sugar export to the cytoplasm and result in an increase in starch production when silenced individually. Simultaneous silencing of DPE1 and DPE2 in Nicotiana benthamiana resulted in a near complete suppression in starch and accumulation of malto-oligosaccharides.

References

Feb 13, 2001·The Plant Journal : for Cell and Molecular Biology·F RatcliffD C Baulcombe
Mar 3, 2001·Current Opinion in Plant Biology·N Bouché, D Bouchez
May 22, 2001·The Plant Journal : for Cell and Molecular Biology·J H CritchleyS M Smith
Mar 14, 2002·Plant Physiology·Samuel C ZeemanAlison M Smith
May 25, 2002·The Plant Journal : for Cell and Molecular Biology·Yule LiuS P Dinesh-Kumar
Dec 7, 2002·The Plant Cell·Allen SessionsStephen A Goff
Mar 5, 2004·The Plant Journal : for Cell and Molecular Biology·Tansy ChiaAlison M Smith
May 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Wolfram WeckwerthOliver Fiehn
Jul 1, 2004·The Plant Journal : for Cell and Molecular Biology·Gianinna BrignetiJonathan D G Jones
Sep 15, 2005·The New Phytologist·Lee J Sweetlove, Alisdair R Fernie
Apr 28, 2006·The Plant Journal : for Cell and Molecular Biology·Joerg FettkeMartin Steup
Sep 4, 2008·The Plant Journal : for Cell and Molecular Biology·Shai NashilevitzAvraham A Levy
Jan 12, 2010·Nature Structural & Molecular Biology·Sergej DjuranovicRachel Green
Mar 3, 2010·Annual Review of Plant Biology·Samuel C ZeemanAlison M Smith
Dec 25, 2010·The Plant Cell·Allison Mallory, Hervé Vaucheret

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