Virus-induced p38 MAPK activation facilitates viral infection

Theranostics
Yuting ChengZhijian Cao

Abstract

Rationale: Many viral infections are known to activate the p38 mitogen-activated protein kinase (MAPK) signaling pathway. However, the role of p38 activation in viral infection and the underlying mechanism remain unclear. The role of virus-hijacked p38 MAPK activation in viral infection was investigated in this study. Methods: The correlation of hepatitis C virus (HCV) infection and p38 activation was studied in patient tissues and primary human hepatocytes (PHHs) by immunohistochemistry and western blotting. Coimmunoprecipitation, GST pulldown and confocal microscopy were used to investigate the interaction of p38α and the HCV core protein. In vitro kinase assays and mass spectrometry were used to analyze the phosphorylation of the HCV core protein. Plaque assays, quantitative real time PCR (qRT-PCR), western blotting, siRNA and CRISPR/Cas9 were used to determine the effect of p38 activation on viral replication. Results: HCV infection was associated with p38 activation in clinical samples. HCV infection increased p38 phosphorylation by triggering the interaction of p38α and TGF-β activated kinase 1 (MAP3K7) binding protein 1 (TAB1). TAB1-mediated p38α activation facilitated HCV replication, and pharmaceutical inhibition of p3...Continue Reading

Citations

Apr 4, 2021·International Journal of Molecular Sciences·Bingting YuMaikel P Peppelenbosch
Apr 4, 2021·Journal of Clinical Medicine·Alessia VirzìJoachim Lupberger
May 1, 2021·International Journal of Molecular Sciences·Jeremy C BurtonNeil J Grimsey
Jun 3, 2021·Biology·Kirill I YurlovAlla A Kushch
Oct 7, 2021·PLoS Pathogens·Sushil KhatiwadaDaniel L Rock

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Methods Mentioned

BETA
PMA
PCR
Transfection
Protein Assay
Assay
immunoprecipitation
pulldown
coimmunoprecipitation
confocal microscopy

Software Mentioned

GraphPad Prism6
Adobe Photoshop
ImageJ

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