Virus-like particles induce MHC class I-restricted T-cell responses. Lessons learned from the hepatitis B small surface antigen

Intervirology
Reinhold SchirmbeckJörg Reimann

Abstract

H-2d mice generated a major histocompatibility complex (MHC) class I (Ld)-restricted T-cell response of defined restriction and epitope specificity to the hepatitis B virus small surface antigen (HBsAg). Here, we compare different vaccination techniques that prime in vivo class I-restricted, murine cytotoxic T lymphocyte (CTL) precursors and specific serum antibody responses. CTL were efficiently primed by the injection of low doses of recombinant native HBsAg particles without adjuvants, by the injection of low doses of denatured HBsAg monomers without adjuvants, by infection with recombinant vaccinia virus carrying a HBsAg-encoding gene, or by intramuscular transfer of plasmid DNA encoding HBsAg under appropriate promoter control. The observation that the injection of 100 ng to 1 microgram of native HBsAg "virus-like particles' (VLP) without adjuvants is an exogenous antigen preparation that efficiently primes class I-restricted CTL responses was unexpected. It reveals a novel aspect of the immunogenicity of VLP for T cells.

Citations

Jul 9, 1999·Reviews in Medical Virology·C Porta, G P Lomonossoff
Oct 7, 2009·Expert Review of Vaccines·Franco Maria BuonaguroLuigi Buonaguro
Nov 30, 2018·Biotechnology Journal·Sandiswa MbewanaEdward P Rybicki
Jan 18, 2011·Journal of Acquired Immune Deficiency Syndromes : JAIDS·David FrancoDavid D Ho
Oct 18, 2011·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Simon TrowitzschImre Berger
Feb 19, 2013·Expert Review of Vaccines·Luigi BuonaguroFranco M Buonaguro
Feb 13, 2016·Clinical and Experimental Vaccine Research·Hyunil KimKi Myung Choi
Nov 3, 2011·Expert Review of Vaccines·Luigi BuonaguroFranco M Buonaguro
Nov 9, 2017·Advanced Healthcare Materials·Kelly M HainlineJoel H Collier
Jul 26, 2013·Nature Reviews. Immunology·Douglas M SmithJames R Baker
Dec 10, 2014·Expert Review of Vaccines·Vidadi YusibovStephen J Streatfield
Sep 20, 2017·Vaccines·Bonto FaburayJuergen A Richt
Jun 19, 2001·Biological Chemistry·H MollenkopfS H Kaufmann
Oct 30, 2020·Frontiers in Immunology·Chun-Wei ChenJoan Joseph-Munné
Oct 27, 2020·Frontiers in Immunology·Morgan BrisseHinh Ly
Mar 19, 2021·Biotechnology Reports·Maryam Moradi VahdatAli Niazi
Jul 21, 1997·Virology·M MüllerL Gissmann
Apr 1, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·S BabiukM Foldvari

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.