Virus Like Particles (VLP) as multivalent vaccine candidate against Chikungunya, Japanese Encephalitis, Yellow Fever and Zika Virus.

Scientific Reports
Himanshu GargAnjali Joshi

Abstract

Mosquito borne viral diseases are an emerging threat as evident from the recent outbreak of Zika virus (ZIKV) as well as repeated outbreaks of Chikungunya (CHIKV), Yellow fever (YFV) and Japanese encephalitis (JEV) virus in different geographical regions. These four arboviruses are endemic in overlapping regions due to the co-prevalence of the transmitting mosquito vector species Aedes and Culex. Thus, a multivalent vaccine that targets all four viruses would be of benefit to regions of the world where these diseases are endemic. We developed a potential Virus Like Particle (VLP) based multivalent vaccine candidate to target these diseases by using stable cell lines that continuously secrete VLPs in the culture supernatants. Moreover, inclusion of Capsid in the VLPs provides an additional viral protein leading to an enhanced immune response as evident from our previous studies with ZIKV. Immunization of Balb/c mice with different combinations of Capsid protein containing VLPs either as monovalent, bivalent or tetravalent formulation resulted in generation of high levels of neutralizing antibodies. Interestingly, the potential tetravalent VLP vaccine candidate provided strong neutralizing antibody titers against all four viruses...Continue Reading

References

Apr 1, 1995·Journal of Virology·V F Yamshchikov, R W Compans
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M Lobigs
Sep 18, 2002·Archives of Medical Research·Duane J Gubler
Sep 19, 2003·Trends in Microbiology·Rob Noad, Polly Roy
Oct 28, 2009·Antiviral Research·Scott C Weaver, William K Reisen
Sep 9, 2011·The Journal of General Virology·Karen L MansfieldAnthony R Fooks
Feb 19, 2013·Expert Review of Vaccines·Joshua W Wang, Richard B S Roden
Oct 29, 2013·Human Vaccines & Immunotherapeutics·Sang-Im Yun, Young-Min Lee
Feb 11, 2014·Current Protocols in Microbiology·James D BrienMichael S Diamond
Feb 19, 2015·The New England Journal of Medicine·Elmar A JouraUNKNOWN Broad Spectrum HPV Vaccine Study
May 20, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S Pils, E A Joura
Jun 25, 2015·The New England Journal of Medicine·Elmar JouraAlain Luxembourg
Jun 25, 2015·The New England Journal of Medicine·Patrick Murray
Sep 15, 2015·Expert Review of Vaccines·Andrew S Beck, Alan Dt Barrett
Feb 13, 2016·Travel Medicine and Infectious Disease·Remi N Charrel
Mar 11, 2016·Journal of Virology·Helen M Lazear, Michael S Diamond
May 26, 2016·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Hamid SalimiRobyn S Klein
Dec 7, 2016·The Journal of Infectious Diseases·Sergio YactayoPilar Ramon-Pardo
May 5, 2017·Viruses·Michael R Holbrook
Jul 30, 2017·Clinical Therapeutics·Matthew H Collins, Stefan W Metz
Aug 11, 2017·Journal of Virology·Himanshu GargAnjali Joshi
Sep 10, 2017·Seminars in Immunology·Mona O MohsenMartin F Bachmann
May 31, 2018·Advances in Experimental Medicine and Biology·Paul R Young

❮ Previous
Next ❯

Citations

Jun 4, 2020·Vaccines·Aryamav PattnaikAsit K Pattnaik
Dec 4, 2020·Viruses·Priscila M S Castanha, Ernesto T A Marques
Feb 4, 2021·Physical Chemistry Chemical Physics : PCCP·Colin P PilkingtonYuval Elani
Apr 17, 2021·International Journal of Biological Macromolecules·Khatereh Asadi, Ahmad Gholami
Jun 1, 2021·Current Opinion in Biotechnology·Michela PulixAlan J Dickson
Jul 3, 2021·Nanomaterials·Ivana RuseskaAndreas Zimmer
Aug 30, 2021·Trends in Pharmacological Sciences·Shruthi Polla RaviArghya Paul
Oct 24, 2021·International Journal of Pharmaceutics·Maria TsakiriCostas Demetzos

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
fluorescence microscopy
flow cytometry
Fluorescence
electron microscopy
Transmission Electron Microscopy
immunoprecipitation
transfections
PCR

Software Mentioned

Multi Gauge
NIS Elements AR
FloJo
GENETOOLS
DNAStar

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.