PMID: 6406686Jun 1, 1983Paper

Virus receptor interaction in the adenovirus system. II. Capping and cooperative binding of virions on HeLa cells

Journal of Virology
R PerssonE Everitt

Abstract

Adenovirus type 2 attachment to HeLa cells was analyzed under controlled conditions. The temperature-dependent attachment kinetics revealed an inflection point at around 20 degrees C, and above this temperature the increase of the rate was doubled. In multiplicity dependence experiments, the attachment exhibited positive cooperative binding at 37 degrees C. This binding pattern was inhibited by low temperatures and prefixation of cells with 0.015% glutaraldehyde. Attachment of rhodamine-labeled virions revealed capping of the particles on 15% of the cells at 37 degrees C. Capping was inhibited by low temperatures, glutaraldehyde fixation of cells, and treatment with cytochalasin B, azide, and 2-deoxyglucose. Consequently, we propose that the adenovirus type 2 attachment to cells leads to rearrangements in the plasma membrane, resulting in cooperative binding and capping of the virus particles.

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Citations

Apr 4, 2009·PLoS Pathogens·Stephanie Abromaitis, Richard S Stephens
Nov 24, 2019·FEBS Letters·Noémie Pied, Harald Wodrich
Jul 15, 2006·Journal of Neurocytology·Giri VenkatramanFrank L Margolis
Jun 29, 2007·Biotechnology and Bioengineering·Philippe-Alexandre GilbertAlain Garnier
Oct 24, 2000·Journal of Virology·D M Shayakhmetov, A Lieber
Jul 27, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·M A SeidmanP L Leopold
Feb 17, 2000·Journal of Structural Biology·M Y Nakano, U F Greber
Nov 1, 1991·Journal of Virology·M J VargaE Everitt
Nov 1, 1994·Journal of Virology·J HottaH S Ginsberg
Sep 1, 1984·Journal of Virology·U Svensson, R Persson
Jul 1, 1985·Journal of Virology·U BrüggemannW Doerfler
Nov 1, 1996·Journal of Virology·N MitterederB C Trapnell

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