Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 Receptor

BioRxiv : the Preprint Server for Biology
Peng ZhaoLance Wells

Abstract

The current COVID-19 pandemic is caused by the SARS-CoV-2 betacoronavirus, which utilizes its highly glycosylated trimeric Spike protein to bind to the cell surface receptor ACE2 glycoprotein and facilitate host cell entry. We utilized glycomics-informed glycoproteomics to characterize site-specific microheterogeneity of glycosylation for a recombinant trimer Spike mimetic immunogen and for a soluble version of human ACE2. We combined this information with bioinformatic analyses of natural variants and with existing 3D-structures of both glycoproteins to generate molecular dynamics simulations of each glycoprotein alone and interacting with one another. Our results highlight roles for glycans in sterically masking polypeptide epitopes and directly modulating Spike-ACE2 interactions. Furthermore, our results illustrate the impact of viral evolution and divergence on Spike glycosylation, as well as the influence of natural variants on ACE2 receptor glycosylation that, taken together, can facilitate immunogen design to achieve antibody neutralization and inform therapeutic strategies to inhibit viral infection.

Citations

Oct 3, 2020·Frontiers in Molecular Biosciences·Giulia PalermoValentina Tozzini
Nov 28, 2020·Journal of Clinical Immunology·Cristiane J Nunes-SantosSergio D Rosenzweig
Jan 10, 2021·Nature Communications·Hin ChuKwok-Yung Yuen

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Methods Mentioned

BETA
glycosylation
electron microscopy
several

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