Visceral clear cell sarcoma of soft tissue with confirmation by EWS-ATF1 fusion detection

Ultrastructural Pathology
Laura GranvilleDolores H López-Terrada

Abstract

Clear cell sarcoma of soft tissue (CCS-ST) is a rare malignant neoplasm characterized by a tumor-defining translocation [t(12;22) (q13;q12)], resulting in the EWS-ATF1 gene fusion. An extremely limited number of visceral CCS-ST cases have been reported in the literature. Here the authors report a visceral CCS-ST in a Hispanic adolescent male with a large infiltrative mass involving the small bowel. The tumor was evaluated by light microscopy, immunocytochemistry, electron microscopy, cytogenetics, and molecular genetics. The tumor cells were strongly positive for S-100 protein, but negative for HMB-45. Rare premelanosomes were identified only after an extensive search with electron microscopy. Cytogenetics showed a characteristic t(12;22)(q13;q12) for CCS-ST with isochromosome 18q and trisomy 22. An EWS exon 8 sense primer and an antisense ATF1 primer were employed for detection of the CCS-ST tumor-defining EWS-ATF1 translocation, using reverse transcriptase-polymerase chain reaction techniques (RT-PCR), and the fusion gene breakpoint underwent DNA sequencing. This tumor is exceptional, because it is the first visceral CCS-ST that has been confirmed by RT-PCR and DNA sequencing. This case also illustrates the necessity of a mul...Continue Reading

References

Jan 9, 1998·The American Journal of Surgical Pathology·L R DonnerS Dobin
Jun 10, 2000·Pathology International·T FukudaT Suzuki
May 7, 2002·International Journal of Cancer. Journal International Du Cancer·Ioannis PanagopoulosNils Mandahl
Dec 4, 2002·Histopathology·P PauwelsP C W Hogendoorn
Jul 24, 2003·The Journal of Molecular Diagnostics : JMD·Chung-Che Chang, Vinod B Shidham

❮ Previous
Next ❯

Citations

May 11, 2012·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Rita AlaggioSara O Vargas
Dec 26, 2006·Cancer Letters·Karolin H HallorIoannis Panagopoulos
May 13, 2009·Annals of Diagnostic Pathology·Roberto TiraboscoAdrienne M Flanagan
Feb 28, 2015·Archives of Pathology & Laboratory Medicine·Jayson Wang, Khin Thway
Jan 15, 2017·Diagnostic Pathology·Long JinShuguang Liu
Oct 5, 2007·International Journal of Surgical Pathology·Barton KenneyLarisa Davydova
Apr 18, 2008·International Journal of Surgical Pathology·Ihab AbdulkaderJerónimo Forteza
Apr 7, 2018·Journal of Gastrointestinal Cancer·Gokce AskanOlca Basturk
Jul 20, 2007·Virchows Archiv : an International Journal of Pathology·Camilla E CominLuca Messerini
Apr 15, 2008·The American Journal of Surgical Pathology·Pamela L LyleWilliam A Robinson
Apr 8, 2009·Pediatric Blood & Cancer·Joanne P LagmayPeter Baker
Dec 31, 2011·International Journal of Surgical Pathology·Francesco E D'AmicoNicolò Bassi
May 20, 2015·World Journal of Gastroenterology : WJG·Sung Bum KimMi Jin Gu
Aug 26, 2014·International Journal of Surgical Pathology·Gülçin YegenYersu Kapran
Aug 4, 2012·Journal of Pediatric Hematology/oncology·Susumu InoueLori McQuillan
Nov 16, 2011·International Journal of Surgical Pathology·Patrick ShenjereSaumitra S Banerjee
Jul 18, 2015·Clinical Journal of Gastroenterology·Daisuke IshiiShiro Nakano
Apr 14, 2017·World Journal of Gastroenterology : WJG·Hao SuHai-Tao Zhou
Feb 2, 2021·Journal of Gastrointestinal Cancer·Aditi DamleRoopa Rachel Paulose

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.