Viscoelastic behavior of mammalian DNA

Biophysical Journal
E S Chase, R H Shafer

Abstract

The viscoelastic behavior of rat 9L cellular DNA was studied as a function of the detergent used for lysis, the pH and duration of lysis, and gamma ray dose. For nondenaturing lysis conditions, a model of the DNA was proposed to account for the effects of these agents on the viscoelastic retardation time. It was concluded that these agents affect the hydrodynamic radius of the DNA rather than its molecular weight. For denaturing lysis conditions, molecular weights calculated from the relaxation time were consistent with those calculated from alkaline sucrose sedimentation profiles.

References

Apr 1, 1986·Biulleten' eksperimental'noĭ biologii i meditsiny·S E BreslerE I Shvarts
Sep 3, 1998·Mutation Research·M V FilatovL A Noskin
Jan 15, 2000·Journal of Biomolecular Structure & Dynamics·J Y OstashevskyC S Lange

Related Concepts

Pathologic Cytolysis
RNA Conformation
Viscosity
Sedimentation Procedure
Sucrose
Elasticity
Hydrogen-Ion Concentration
Brain Tumor, Recurrent
Saccharum officinale, sucrose, cane sugar, Homeopathic preparation
Lysis

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