Viscosin-like lipopeptides from frog skin bacteria inhibit Aspergillus fumigatus and Batrachochytrium dendrobatidis detected by imaging mass spectrometry and molecular networking

Scientific Reports
Christian Martin HMarcelino Gutiérrez

Abstract

Amphibian populations worldwide have declined and in some cases become extinct due to chytridiomycosis, a pandemic disease caused by the fungus Batrachochytrium dendrobatidis; however, some species have survived these fungal epidemics. Previous studies have suggested that the resistance of these species is due to the presence of cutaneous bacteria producing antifungal metabolites. As our understanding of these metabolites is still limited, we assessed the potential of such compounds against human-relevant fungi such as Aspergillus. In this work we isolated 201 bacterial strains from fifteen samples belonging to seven frog species collected in the highlands of Panama and tested them against Aspergillus fumigatus. Among the 29 bacterial isolates that exhibited antifungal activity, Pseudomonas cichorii showed the greatest inhibition. To visualize the distribution of compounds and identify them in the inhibition zone produced by P. cichorii, we employed MALDI imaging mass spectrometry (MALDI IMS) and MS/MS molecular networking. We identified viscosin and massetolides A, F, G and H in the inhibition zone. Furthermore, viscosin was isolated and evaluated in vitro against A. fumigatus and B. dendrobatidis showing MIC values of 62.50 µ...Continue Reading

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Citations

Dec 28, 2019·Marine Drugs·Sébastien DuperronMarie-Lise Bourguet-Kondracki
Feb 10, 2021·Nature Ecology & Evolution·Ty N F RoachCrawford Drury
May 9, 2021·Current Opinion in Microbiology·Louise A Rollins-Smith, Emily H Le Sage
Sep 11, 2021·Environmental Science and Pollution Research International·Guilherme Henrique CarrascoLia Raquel de Souza Santos

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Datasets Mentioned

BETA
KX456226-KX456434

Methods Mentioned

BETA
amplicon sequencing
NMR
electrophoresis

Software Mentioned

Geneious
BLASTn
Cytoscape
GNPS
Global Natural Products Social Molecular Networking online pla...
MS
ImageJ
ITOL
Clustering
MZmine

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