PMID: 3768476Aug 1, 1986Paper

Viscosity of concentrated solutions and of human erythrocyte cytoplasm determined from NMR measurement of molecular correlation times. The dependence of viscosity on cell volume

Biophysical Chemistry
Z H Endre, P W Kuchel

Abstract

Metabolically active human erythrocytes were incubated with [alpha-13C]glycine which led to the specific enrichment of intracellular glutathione. The cells were then studied using 13C-NMR in which the longitudinal relaxation times (T1) and nuclear Overhauser enhancements of the free glycine and glutathione were measured. The T1 values of labelled glycine were also determined in various-concentration solutions of bovine serum albumin and glycerol and also of the natural abundance 13C of glycerol in glycerol solutions. From the T1 estimates the rotational correlation time (tau r) was calculated using a formula based on a model of an isotropic spherical rotor or that of a symmetrical ellipsoidal rotor; for glycine the differences in estimates of tau r obtained using the two models were not significant. From the correlation times and by use of the Stokes-Einstein equations viscosity and translational diffusion coefficients were calculated; thus comment can be made on the likelihood of diffusion control of certain enzyme-catalysed reactions in the erythrocyte. Bulk viscosities of the erythrocyte cytoplasm and the above-mentioned solutions were measured using Ostwald capillary viscometry. Large differences existed between the latter ...Continue Reading

References

Aug 22, 1977·Biochemical and Biophysical Research Communications·P D Morse
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Citations

Jul 12, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jonathan V SehyJeffrey J Neil
Sep 5, 2002·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jeffrey M MacdonaldThomas L James
Jul 1, 1990·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·M A MartinR L Armstrong
Feb 1, 1990·NMR in Biomedicine·K Kirk
May 31, 2012·European Biophysics Journal : EBJ·Timothy J LarkinPhilip W Kuchel
Feb 1, 1994·International Journal of Biological Macromolecules·K Monkos
Feb 1, 1996·International Journal of Biological Macromolecules·K Monkos
Aug 16, 2013·NMR in Biomedicine·Irene Marco-RiusKevin M Brindle
Jan 1, 1990·Critical Reviews in Oncology/hematology·T ShigaK Kon
Sep 30, 2006·Biophysical Chemistry·Silvia ZorrillaM Pilar Lillo
May 1, 1988·British Journal of Haematology·J Stuart, J C Ellory
Jan 1, 1990·Critical Reviews in Clinical Laboratory Sciences·J Stuart, G B Nash
Mar 24, 2016·Magnetic Resonance in Chemistry : MRC·Timothy J LarkinKonstantin I Momot
Jul 1, 1989·Biophysical Chemistry·W S PriceB A Cornell
Mar 11, 1988·Biochimica Et Biophysica Acta·M Barac-NietoR K Gupta

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