Visfatin effects on telomerase gene expression in AGS gastric cancer cell line

Indian Journal of Cancer
M MohammadiM Mohaddes

Abstract

The aim of this study was to assess visfatin expression and its effect on human telomerase gene expression in AGS gastric cancer cell line. In this study, human gastric cancer (AGS) cell line was established as an in vitro model. Reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay was performed to show that visfatin expression in messenger ribonucleic acid (mRNA) and protein level respectively. After stimulating with increasing concentrations of visfatin for times of 24 h and 48 h, cell proliferation was assessed by 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) assay. In order to investigate telomerase gene expression affected by visfatin in AGS cell line, total RNA was extracted and complementary deoxyribonucleic acid was synthesized buy using commercially available kits. Expression of human telomerase reverse transcriptase (hTERT) mRNA was carried out by real-time RT-PCR. After visfatin treatment gastric cell line proliferation was enhanced and was increased the expression of hTERT. The obtained data showed that visfatin induces endogenously gastric cancer cell proliferation and increases telomerase (hTERT) gene expression, as a cancer gene. Based on ...Continue Reading

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Citations

Apr 30, 2016·British Journal of Pharmacology·Ambra A GrollaJaswinder K Sethi
Nov 25, 2018·Journal of Cellular Physiology·Masoumeh MohammadiHassan Mehrad-Majd
Jan 28, 2020·International Journal of Molecular Medicine·Zhaoyun LiuRong Fu
Nov 13, 2020·Clinical and Experimental Gastroenterology·Khadijeh Kalan FarmanfarmaHamid Salehiniya

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