VISFATIN (NAMPT) Improves In Vitro IGF1-Induced Steroidogenesis and IGF1 Receptor Signaling Through SIRT1 in Bovine Granulosa Cells

Biology of Reproduction
Maxime ReverchonJoëlle Dupont

Abstract

VISFATIN is a novel adipokine, also known as a nicotinamide phosphorybosyltransferase (NAMPT), that is able to modulate different processes, including lipid and glucose metabolism, oxidative stress, inflammation, and insulin resistance. Recent data suggest that it also plays a role in reproductive function in rats, humans, and chickens. Here we identified VISFATIN in the bovine ovary and investigated the in vitro effects of this hormone on granulosa cell steroidogenesis and proliferation and oocyte maturation. By RT-PCR, immunoblotting, and immunohistochemistry, we found VISFATIN in various ovarian cells, including granulosa and theca cells, corpus luteum, and oocytes. In cultured bovine granulosa cells, we showed that IGF1 (10(-8) M) and VISFATIN (10 and 100 ng/ml) but not FSH (10(-8) M) increased mRNA expression levels of NAMPT after 48 h of stimulation. Moreover, we observed that human recombinant VISFATIN (hVisf, 10 ng/ml, 48 h) increased the release of progesterone and estradiol secretion, and this was associated with an increase in the protein level of STAR, the HSD3B activity, and the phosphorylation levels of IGF1R and MAPK ERK1/2 in the presence or absence of IGF1 (10(-8) M). All these effects were abolished when NAMPT...Continue Reading

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Citations

Mar 3, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·J RocheJ Dupont
Jan 2, 2018·World Journal of Gastroenterology : WJG·Ferenc SiposGyörgyi Műzes
May 23, 2020·International Journal of Molecular Sciences·Anthony EstienneJoëlle Dupont
Mar 29, 2020·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Anna WnukDariusz Szukiewicz
Feb 14, 2018·Molecular Medicine Reports·Piotr CelichowskiMarcin Ruciński
Jul 19, 2018·International Journal of Endocrinology·Namya MelloukJoëlle Dupont
Dec 12, 2020·International Journal of Molecular Sciences·Patrycja KurowskaAgnieszka Rak
Nov 27, 2020·Frontiers in Cardiovascular Medicine·Alexander E BerezinMichael Lichtenauer
Feb 22, 2021·The Journal of Steroid Biochemistry and Molecular Biology·Saba NikanfarRalf Dittrich
Mar 26, 2021·Human Reproduction Update·Sebastião Freitas de MedeirosRobert John Norman
Jun 11, 2021·Journal of Agricultural and Food Chemistry·Lingyu ZhangYulong Yin
Jun 26, 2021·Molecular and Cellular Endocrinology·Ophélie BernardiJoëlle Dupont
Jul 3, 2021·Reproduction, Fertility, and Development·Jennifer L JuengelPeter Smith

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