Visible-Light-Induced Effects of Au Nanoparticle on Laccase Catalytic Activity

ACS Applied Materials & Interfaces
Sijie GuoZhenhui Kang

Abstract

A deep understanding of the interaction between the nanoparticle and enzyme is important for biocatalyst design. Here, we report the in situ synthesis of laccase-Au NP (laccase-Au) hybrids and its catalytic activity modulation by visible light. In the present hybrid system, the activity of laccase was significantly improved (increased by 91.2% vs free laccase) by Au NPs. With a short time visible light illumination (λ > 420 nm, within 3 min), the activity of laccase-Au hybrids decreased by 8.1% (vs laccase-Au hybrid without light), which can be restored to its initial one when the illumination is removed. However, after a long time illumination (λ > 420 nm, over 10 min), the catalytic activity of laccase-Au hybrids consecutively decreases and is not reversible even after removing the illumination. Our experiments also suggested that the local surface plasma resonance effect of Au NPs causes the structure change of laccase and local high temperature near the Au NPs. Those changes eventually affect the transportation of electrons in laccase, which further results in the declined activity of laccase.

References

Nov 7, 1996·Chemical Reviews·Edward I. SolomonTimothy E. Machonkin
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Ravi ShuklaMurali Sastry
May 24, 2006·Biotechnology Advances·Susana Rodríguez Couto, José Luis Toca Herrera
Mar 14, 2007·Biochemical and Biophysical Research Communications·Sisir DebnathPrasanta Kumar Das
Nov 13, 2007·Journal of the American Chemical Society·Akihiro FurubeMasanori Tachiya
Jun 24, 2008·Accounts of Chemical Research·Sara E SkrabalakYounan Xia
Apr 10, 2009·Journal of Hazardous Materials·Kumarasamy MurugesanYoon-Seok Chang
Sep 8, 2010·Chemical Society Reviews·Claire M CobleyYounan Xia
Feb 3, 2012·Advanced Materials·Pamela Torres-SalasFrancisco J Plou
Jun 5, 2012·Nature Nanotechnology·Jun GeRichard N Zare
Dec 15, 2012·ACS Nano·Matthew D BlankschienMichael S Wong
Aug 16, 2013·Chemical Communications : Chem Comm·Xinjian YangXiaogang Qu
Oct 28, 2014·ACS Applied Materials & Interfaces·Liping FangBai Yang

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Citations

Aug 6, 2016·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Mark D Cannatelli, Arthur J Ragauskas
Mar 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhixian LeiZian Lin
Jun 28, 2019·Advanced Synthesis & Catalysis·Christiane ClaaßenDörte Rother
Oct 21, 2020·Chemical Communications : Chem Comm·Vasantha Krishna KadambarArtem Melman
Jul 10, 2020·Biochemistry·Heloise Ribeiro de BarrosLuis M Liz-Marzán

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