Visible micro-Raman spectroscopy of single human mammary epithelial cells exposed to x-ray radiation

Journal of Biomedical Optics
Ines DelfinoMaria Lepore

Abstract

A micro-Raman spectroscopy investigation has been performed in vitro on single human mammary epithelial cells after irradiation by graded x-ray doses. The analysis by principal component analysis (PCA) and interval-PCA (i-PCA) methods has allowed us to point out the small differences in the Raman spectra induced by irradiation. This experimental approach has enabled us to delineate radiation-induced changes in protein, nucleic acid, lipid, and carbohydrate content. In particular, the dose dependence of PCA and i-PCA components has been analyzed. Our results have confirmed that micro-Raman spectroscopy coupled to properly chosen data analysis methods is a very sensitive technique to detect early molecular changes at the single-cell level following exposure to ionizing radiation. This would help in developing innovative approaches to monitor radiation cancer radiotherapy outcome so as to reduce the overall radiation dose and minimize damage to the surrounding healthy cells, both aspects being of great importance in the field of radiation therapy.

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Citations

Feb 18, 2016·Scientific Reports·Samantha J HarderAndrew Jirasek
Nov 8, 2017·Journal of Biomedical Optics·Fabrizia d'ApuzzoCarlo Camerlingo
Aug 25, 2018·Medical Physics·Patricia A K Oliver, Rowan M Thomson
Nov 30, 2018·International Journal of Radiation Biology·Maria LasalviaVito Capozzi
Mar 28, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Maciej RomanWojciech M Kwiatek

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