Visinin-like 1 is upregulated in aldosterone-producing adenomas with KCNJ5 mutations and protects from calcium-induced apoptosis

Hypertension
Tracy Ann WilliamsPaolo Mulatero

Abstract

Visinin-like 1 (VSNL1) is upregulated in aldosterone-producing adenomas (APAs) compared with normal adrenals. We demonstrate that VSNL1 overexpression in adrenocortical carcinoma cells (NCI H295R) upregulates basal and angiotensin II-stimulated CYP11B2 gene expression 3.2- and 1.5-fold, respectively. Conversely, silencing VSNL1 by RNA interference decreases angiotensin II-stimulated CYP11B2 expression and aldosterone secretion by 41.0% and 34.5%, respectively. Mutations in the potassium channel KCNJ5 have been identified in APAs that result in sodium influx and membrane depolarization and are postulated to result in calcium influx in adrenal glomerulosa cells. VSNL1 and CYP11B2 are 8.1- and 6.0-fold more highly expressed, respectively, in APAs harboring KCNJ5 mutations compared with those without, and the upregulation of VSNL1 in these APAs accounts for the overexpression of VSNL1 in the total APA sample set compared with normal adrenals. Silencing VSNL1 in H295R cells renders them sensitive to ionomycin-induced apoptosis, indicating that VSNL1 protects these cells against calcium-induced cell death. Concomitant expression of mutated KCNJ5 (G151R) and silencing VSNL1 results in apoptosis of H295R cells, an effect that is blocke...Continue Reading

References

Jul 3, 2003·Nature Reviews. Molecular Cell Biology·Sten OrreniusPierluigi Nicotera
Sep 8, 2004·Biochemical and Biophysical Research Communications·Paul GierkeKarl-Heinz Braunewell
Mar 22, 2006·The Journal of Cell Biology·Tomoe Y NakamuraJunichi Nabekura
Apr 25, 2008·Nature Reviews. Cancer·H Llewelyn Roderick, Simon J Cook
May 29, 2008·The Journal of Clinical Endocrinology and Metabolism·David S GellerRichard P Lifton
Jun 17, 2008·The Journal of Clinical Endocrinology and Metabolism·John W FunderUNKNOWN Endocrine Society
May 16, 2009·Current Hypertension Reports·Paolo MulateroFranco Veglio
Jan 18, 2011·The Journal of Clinical Endocrinology and Metabolism·Bruno Ferraz-de-SouzaJohn C Achermann
Feb 1, 2011·Reviews in Endocrine & Metabolic Disorders·Paolo MulateroFranco Veglio
Jan 26, 2012·Hypertension·Sheerazed BoulkrounMaria-Christina Zennaro

❮ Previous
Next ❯

Citations

Sep 23, 2014·Molecular and Cellular Endocrinology·Silvia MonticoneWilliam E Rainey
Nov 1, 2012·Annales d'endocrinologie·A Al-SalamehP F Plouin
Jul 9, 2013·Clinical and Experimental Pharmacology & Physiology·Carolina Velarde-MirandaCelso E Gomez-Sanchez
Jan 3, 2015·Current Opinion in Pharmacology·Livia Lenzini, Gian Paolo Rossi
Nov 22, 2012·Molecular and Cellular Endocrinology·Enzo LalliIsabelle Mus-Veteau
Feb 5, 2013·Molecular and Cellular Endocrinology·Sheerazed BoulkrounMaria-Christina Zennaro
Dec 3, 2014·Molecular and Cellular Endocrinology·Sachiko Konosu-FukayaHironobu Sasano
Apr 10, 2015·High Blood Pressure & Cardiovascular Prevention : the Official Journal of the Italian Society of Hypertension·Jacopo BurrelloPaolo Mulatero
May 4, 2016·High Blood Pressure & Cardiovascular Prevention : the Official Journal of the Italian Society of Hypertension·Valentina CrudoPaolo Mulatero
Sep 30, 2016·Endocrine Journal·Takashi OkamuraMasanobu Yamada
Apr 13, 2017·Journal of Molecular Endocrinology·Fabio Luiz Fernandes-RosaMaria-Christina Zennaro
Dec 12, 2012·Nature Reviews. Endocrinology·Paolo MulateroTracy Ann Williams
Nov 27, 2014·The Journal of Endocrinology·Maria-Christina ZennaroFabio Fernandes-Rosa
Jan 15, 2014·Hypertension·Celso E Gomez-Sanchez
Nov 18, 2015·Hypertension·Tracy Ann WilliamsMartin Reincke
Jul 18, 2012·Hypertension·Maria-Christina ZennaroSheerazed Boulkroun
Oct 2, 2013·Hypertension·Tracy Ann WilliamsPaolo Mulatero
Sep 28, 2017·Hypertension·Teresa M SecciaGian Paolo Rossi
Aug 8, 2018·The Journal of Clinical Endocrinology and Metabolism·Kang-Yung PengKwan-Dun Wu
Aug 27, 2019·Hypertension·Yuhong YangTracy Ann Williams
Nov 20, 2016·Journal of Internal Medicine·T ÅkerströmP Hellman
Apr 13, 2018·International Journal of Molecular Sciences·Elke Tatjana Aristizabal PradaTracy A Williams
Sep 25, 2020·Endocrine Journal·Kenji Oki, Celso E Gomez-Sanchez
Oct 10, 2020·Journal of the Endocrine Society·Juilee RegeTobias Else
Feb 2, 2021·The Journal of Clinical Endocrinology and Metabolism·Yuta TezukaAdina F Turcu
May 1, 2021·Biomedicines·Kiyotaka ItchoMasayasu Yoneda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adrenocortical Carcinoma

Adrenocortical carcinoma (ACC) is a rare malignancy of the adrenal cortex, associated with a generally dismal prognosis owing to its aggressive behavior. Here are the latest discoveries pertaining to this disease.