Abstract
Foot-and-mouth disease (FMD), which is caused by foot-and-mouth disease virus (FMDV), is a highly contagious tansboundary disease of cloven-hoofed animals and causes devastating economic damages. Accurate, rapid and simple detection of FMDV is critical to containing an FMD outbreak. Recombinase polymerase amplification (RPA) has been explored for detection of diverse pathogens because of its accuracy, rapidness and simplicity. A visible and equipment-free reverse-transcription recombinase polymerase amplification assay combined with lateral flow strip (LFS RT-RPA) was developed to detect the FMDV using primers and LF probe specific for the 3D gene. The FMDV LFS RT-RPA assay was performed successfully in a closed fist using body heat for 15 min, and the products were visible on the LFS inspected by the naked eyes within 2 min. The assay could detect FMDV serotypes O, A and Asia1, and there were no cross-reactions with vesicular stomatitis virus (VSV), encephalomyocarditis virus (EMCV), classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus 2 (PCV2) and pseudorabies virus (PRV). The analytical sensitivity was 1.0 × 102 copies in vitro transcribed FMDV RNA per reaction, ...Continue Reading
References
Jan 5, 2002·The Veterinary Record·S M ReidS Alexandersen
Jun 8, 2002·Journal of the American Veterinary Medical Association·Johnny D CallahanWilliam M Nelson
Aug 15, 2002·Journal of Virological Methods·Scott M ReidSoren Alexandersen
Sep 19, 2002·Biochemical and Biophysical Research Communications·Richard A CollinsAlbert C H Yu
Jul 16, 2003·Journal of Comparative Pathology·S AlexandersenA J M Garland
Feb 3, 2006·Archives of Virology·J P DukesS Alexandersen
Jun 8, 2006·PLoS Biology·Olaf PiepenburgNiall A Armes
Oct 14, 2008·Journal of Virological Methods·Nigel P FerrisMalik Merza
May 17, 2011·PloS One·Paul LaBarreBernhard Weigl
Nov 26, 2011·The Veterinary Journal·Mikidache MadiDonald P King
Aug 21, 2013·Preventive Veterinary Medicine·T J D Knight-Jones, J Rushton
Aug 27, 2013·PloS One·Ahmed Abd El WahedManfred Weidmann
Dec 7, 2013·Veterinary Research·Syed M Jamal, Graham J Belsham
Mar 22, 2014·Journal of Clinical Microbiology·Andrea AebischerMartin Beer
Sep 30, 2014·PloS One·Lorraine LillisDavid S Boyle
Nov 6, 2014·PloS One·Zachary Austin CrannellRebecca Richards-Kortum
Dec 8, 2014·Molecular and Cellular Probes·Rana K DaherMichel G Bergeron
Dec 1, 2015·Transboundary and Emerging Diseases·E L A HowsonV L Fowler
May 11, 2016·Clinical Chemistry·Rana K DaherMichel G Bergeron
Aug 6, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Jianchang WangWanzhe Yuan
Sep 4, 2016·Transboundary and Emerging Diseases·A AmbagalaO Lung
Oct 30, 2016·Molecular and Cellular Probes·Yang YangZhidong Zhang
Feb 9, 2017·Virology Journal·Yang YangZhidong Zhang
Mar 28, 2017·Analytical Chemistry·Rui WangYibin Ying
Aug 16, 2017·Veterinary Parasitology·Y D WuD H Zhou
Aug 16, 2017·BMC Veterinary Research·Jianchang WangWanzhe Yuan
Dec 15, 2017·BMC Veterinary Research·Peili HouHongbin He
Dec 31, 2017·Molecular and Cellular Probes·Peili HouHongbin He
May 1, 2018·Molecular and Cellular Probes·Libing LiuWanzhe Yuan
Jul 17, 2018·Journal of Virological Methods·Jianchang WangWanzhe Yuan
Citations
Sep 8, 2019·BMC Veterinary Research·Jun JiYingzuo Bi
Jul 10, 2019·PloS One·Yao PengAiping Qin
May 27, 2020·Veterinary Medicine and Science·Qiaoyi HanJianchang Wang
Sep 5, 2020·Plant Biotechnology Journal·Jian JiaoXianbo Zheng
Sep 26, 2020·Frontiers in Veterinary Science·Chuan Loo WongWen Siang Tan
May 10, 2020·BMC Veterinary Research·Xiang GaoYonglu Wang
Nov 30, 2020·Poultry Science·Ji ZhangYouxiang Diao
Feb 23, 2021·Frontiers in Microbiology·Jia XieMingyong Luo
Feb 24, 2021·Journal of Virological Methods·Weiwei WangPing Wei
Dec 31, 2021·Analytical Chemistry·Sabrina PetrucciSapna K Deo