Visual, base-specific detection of nucleic acid hybridization using polymerization-based amplification

Analytical Biochemistry
Ryan R HansenChristopher N Bowman

Abstract

Polymerization-based signal amplification offers sensitive visualization of biotinylated biomolecules functionalized to glass microarrays in a manner suitable for point-of-care use. Here we report using this method for visual detection of multiplexed nucleic acid hybridizations from complex media and develop an application toward point mutation detection and single nucleotide polymorphism (SNP) typing. Primer extension reactions were employed to label selectively and universally all complementary surface DNA hybrids with photoinitiators, permitting simultaneous and dynamic photopolymerization from positive sites to 0.5-nM target concentrations. Dramatic improvements in signal ratios between complementary and mismatched hybrids enabled visual discrimination of single base differences in KRAS codon-12 biomarkers.

References

Jul 26, 2002·Nucleic Acids Research·Deirdre O'MearaJoakim Lundeberg
Sep 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Xiao-Bo ZhongDavid C Ward
Aug 17, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gyorgy Csako
Oct 11, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·C Shad ThaxtonChad A Mirkin
Oct 30, 2007·Nature Materials·Hadley D SikesChristopher N Bowman
Jul 29, 2008·Analytical and Bioanalytical Chemistry·Ryan R HansenChristopher N Bowman

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Citations

Mar 27, 2010·Biomacromolecules·Leah M JohnsonChristopher N Bowman
Feb 23, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Heather J AvensChristopher N Bowman
Dec 17, 2015·Chemical Society Reviews·K Kaastrup, H D Sikes
Jan 26, 2010·Journal of Polymer Science. Part A, Polymer Chemistry·Heather J Avens, Christopher N Bowman

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