Visual detection of telomerase activity with a tunable dynamic range by using a gold nanoparticle probe-based hybridization protection strategy

Nanoscale
Jiasi WangXiaogang Qu

Abstract

We developed a novel telomere complementary (TC) oligonucleotide modified AuNP probe (TC-AuNPs) for colorimetric analysis of telomerase activity. The mechanism of this method is that the telomerase reaction products (TRP), which can hybridize with the TC-AuNPs, are able to protect the AuNPs from the aggregation induced by salt. It is demonstrated that the colorimetric method enabled the analysis of the telomerase activity in 1000 HeLa cells with the naked eye, and down to 100 HeLa cells with the aid of UV-Vis spectroscopy. This strategy is not only convenient and sensitive, but also has a tunable dynamic range. The platform is also applicable for the initial screening of a telomerase inhibitor to discover new anticancer drugs.

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Citations

Jan 28, 2016·Chemical Society Reviews·Ruixue DuanFan Xia
Mar 6, 2015·Chemical Society Reviews·Li Wu, Xiaogang Qu
Dec 7, 2017·Nanoscale·H AldewachiP Gardiner
Jul 7, 2020·The Analyst·Hyo Yong KimHyun Gyu Park
Feb 2, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Bing YangYan Jin
Apr 30, 2017·Chemical Science·Wenjing WangJun-Jie Zhu
Apr 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fanyu MengPeng Miao
Jun 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·EswaraVara Prasadarao KomaralaDhirendra Bahadur
Feb 3, 2021·Chemical Communications : Chem Comm·Ruiyuan ZhangXiaowen Xu
Apr 21, 2021·Chemical Communications : Chem Comm·Zhengze YuBo Tang
Oct 25, 2017·Analytical Chemistry·Haitang YangSongqin Liu
Feb 16, 2018·Analytical Chemistry·Xiaowen XuWei Jiang

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